High-multiplicity of Chitinase genes in Streptomyces coelicolor A3 (2)

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概要

• 論文の詳細を見る

• Six different genes for chitinase from ordered cosmids of the chromosome of Streptomyces coelicolor A3 (2) were identified by hybridization, using the chitinase genes from other Streptomyces spp. as probes, and cloned. The genes were sequenced and analyzed. The genes, together with an additional chitinase gene obtained from the data bank, can be classified into either family 18 or family 19 of the glycosyl hydrolase classification. The five chitinases that fall into family 18 show diversity in their multiple domain structures as well as in the amino acid sequences of their catalytic domains. The remaining two chitinases are members of family 19 chitinases, since their C-terminus shares more than 70% identity with the catalytic domain of ChiC of Streptomyces griseus, the sole gene for family 19 chitinase so far found in an organism other than higher plants.

• 社団法人日本農芸化学会の論文
• 1999-04-23

著者

• WATANABE Takeshi

  Department of Molecular Immunology, Medical Institute of Bioregulation, Kyushu University

• Fujii T

  Kyoto Univ. Kyoto

• Fujii T

  Department Of Applied Biological Chemistry The University Of Tokyo

• Ohno Tsuyoshi

  Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University

• Saito Akiko

  Biotechnology Center Toyama Prefecture

• Saito Akiko

  Department Of Applied Biological Chemistry The University Of Tokyo

• Fujii Takeshi

  National Institute for Agro-Environmental Sciences

• Fujii T

  Inst. Biol. Sci. Univ. Tsukuba

• Watanabe T

  Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University

• Fujii T

  Div. Of Bioresource Sci. Graduate School Of Horticulture Chiba Univ.

• Redenbach Matthias

  Department Of Genetics Genome Research Unit Kaiserslautern University

• SAITO Akihiro

  National Institute for Agro-Environmental Sciences

• MIYASHITA Kiyotaka

  National Institute for Agro-Environmental Sciences

• YONEYAMA Tadakatsu

  Institute of Applied Biochemistry, University of Tsukuba

• Miyashita K

  National Institute For Agro-environmental Sciences

• Miyashita Kiyotaka

  National Inst. For Agro-environmental Sciences

• Watanabe Takeshi

  Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University

• Fujii Takeshi

  National Inst. For Agro-environmental Sciences

• Watanabe Takeshi

  Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University

• Yoneyama Tadakatsu

  Institute Of Applied Biochemistry University Of Tsukuba

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