The Role of NADH : Fe(III)EDTA Oxidoreductase in Ethylene Formation from 2-Keto-4-Methylthiobutyrate
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概要
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A mechanism for the formation of ethylene from 2-keto-4-methylthiobutyric acid (KMBA), a deaminated derivative of L-methionine, was studied with NADH : Fe(III)EDTA oxidoreductase purified from Cryptococcus albidus IFO 0939. The characteristics of the reactions catalyzing the NADH : Fe(III)EDTA oxidoreduction and the formation of ethylene were compared and found to be almost identical. A chemical ethylene-forming system, composed of Fe(II)EDTA, KMBA and oxygen, was constructed and the characteristics of the formation of ethylene were compared with those of the enzymatic reaction. Both the enzymatic and the chemical ethylene-forming reactions were strongly inhibited by scavengers of free radicals, such as benzoic acid, hydroquinone and catalase, and both were activated by H_2O_2. From these results, we propose the following mechanism for the formation of ethylene from KMBA. The first step involves the NADH : Fe(III)EDTA oxidoreduction that is catalyzed by NADH : Fe(III)EDTA oxidoreductase, with Fe(II)EDTA always being supplied as the result. Oxidation of Fe(II)EDTA by molecular oxygen yields the superoxide radical anion (O_2^〓) which can undergo the dismutation reaction to form hydrogen peroxide. Hydrogen peroxide in turn can react with Fe^<2+> via the Fenton reaction, generating the hydroxyl radical (OH^-), which can serve as an oxidizing agent in the oxidation of KMBA to ethylene. The role of the activity of NADH : Fe(III)EDTA oxidoreductase in the formation of ethylene from KMBA in vivo is discussed.
- 社団法人日本生物工学会の論文
- 1990-05-25
著者
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Fujii Takao
Department of Applied Life Science, Faculty of Engineering, Sojo University
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TAKAHASHI Masaya
Department of Stem Cell Biology and Regenerative Medicine, Graduate School of Medical Science, Kansa
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OGAWA TAKAHIRA
Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo Universi
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Fujii T
Kyoto Univ. Kyoto
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Takahashl Mika
Laboratory Of Animal Environment Meiji University
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Ogawa T
Kyoto Univ. Kyoto Jpn
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Ogawa Takahiro
Department Of Biological Science And Technology Science University Of Tokyo
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Ogawa T
Department Of Applied Microbial Technology Faculty Of Biotechnology And Life Science Sojo University
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FUKUDA HIDEO
Department of Applied Microbial Technology, Kumamoto Institute of Technology
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TAZAKI MASATO
Industrial Chemistry, The Kumamoto Institute of Technology
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Fukuda H
Kobe Univ. Kobe Jpn
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Fujii Takao
Department Of Applied Life Science Faculty Of Biotechnology And Life Science Sojo University
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Ogawa T
Kumamoto Inst. Technol. Kumamoto Jpn
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Ogawa Takahira
Department Of Applied Microbial Technology Faculty Of Biotechnology And Life Science Sojo University
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Ogawa Takahira
Dept. Appl. Microbial Technology Kumamoto Institute Of Technology
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Takahashi Masaya
Industrial Chemistry The Kumamoto Institute Of Technology
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Fukuda Hideo
Department Of Applied Microbial Technology The Kumamoto Institute Of Technolgy
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Fukuda Hideo
Dept. Appl. Microbial Technology Kumamoto Institute Of Technology
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Takahashi Masaya
Department Of Stem Cell Biology And Regenerative Medicine Graduate School Of Medical Science Kansai
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Fukuda Hideo
Department Of Applied Microbial Technology Kumamoto Institute Of Technology
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Takahashi Masaya
Department Of Electronic Engineering Aichi College Of Technology
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Takahashi Masaya
Department Of Electrical And Electronic Engineering Oita University
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