Purification and Characterization of an Extracellular Chitosanase Produced by Amycolatopsis sp. CsO-2

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概要

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• Extracellular chitosanase produced by Amycolatopsis sp. CsO-2 was purified to homogeneity by precipitation with ammonium sulfate followed by cation exchange chromatography. The molecular weight of the chitosanase was estimated to be about 27,000 using SDS-polyacrylamide gel electrophoresis and gel filtration. The maximum velocity of chitosan degradation by the enzyme was attained at 55℃ when the pH was maintained at 5.3. The enzyme was stable over a temperature range of 0-50℃ and a pH range of 4.5-6.0. About 50% of the initial activity remained after heating at 100℃for 10 min, indicating a thermostable nature of the enzyme. The isoelectric point of the enzyme was bout 8.8. The enzyme degraded chitosan with a range of deacetylation degree from 70% to 100%, but not chitin or CM-cellulose. The most susceptible substrate was 100% deacetylated chitosan. The enzyme degraded glucosamine tetramer to dimer, and pentamer to dimer and trimer, but did not hydrolyze glucosamine dimer and trimer.

• 社団法人日本生物工学会の論文
• 1994-06-25

著者

• ANDO Akikazu

  Department of Biotechnology, Graduate School of Science and Technology, Chiba University

• Fujii T

  Kyoto Univ. Kyoto

• Fujii T

  Department Of Applied Biological Chemistry The University Of Tokyo

• SHINOYAMA HIROFUMI

  Department of Bioresources Science, Graduate School of Science and Technology, Chiba University

• FUJII TAKAAKI

  Department of Bioresources Science, Graduate School of Science and Technology, Chiba University

• Fujii T

  Inst. Biol. Sci. Univ. Tsukuba

• Ando A

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Fujii T

  Div. Of Bioresource Sci. Graduate School Of Horticulture Chiba Univ.

• Ando Akikazu

  Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University

• Ando Akikazu

  Department Of Agricultural Chemistry Chiba University

• OKAJIMA SHOJI

  Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University

• Okajima Shoji

  Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University:department Of Biotechn

• Shinoyama H

  Department Of Advanced Bioresources Science Graduate School Of Science And Technology Chiba Universi

• Shinoyama Hirofumi

  Department Of Agricultural Chemistry Chiba University

• Fujii Takaaki

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• FUJII Takaaki

  Department of Agricultural Chemistry, Chiba University

• Shinoyama Hirofumi

  Department of Advanced Bioresources Science, Graduate School of Science and Technology, Chiba University

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