Two Genes Encoding Fruit Body Lectins of Pleurotus cornucopiae : Sequence Similarity with the Lectin of a Nematode-Trapping Fungus(Biochemistry & Molecular Biology)

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概要

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• Our previous studies on the fruit body lectin of Pleurotus cornucopiae revealed the existence of three isolectins, composed of two homodimers and one heterodimer of 16- and 15-kDa subunits. In this study, two genes encoding the lectins were cloned and characterized. Both genes encoded 144 amino acids and only 5 amino acids were different within the coding region, but the nucleotide sequences of the 5'-upstream and 3'-downstream regions differed extensively. Southern hybridization with gene-specific probes showed that one gene encoded the 16-kDa and the other encoded the 15-kDa subunit. Functional lectins were synthesized in Escherichia coli under the direction of these genes. On SDS-PAGE, the recombinant lectins showed the same banding patterns as the native lectins. In amino acid sequence, these lectins showed extensive similarity with the lectin from a nematode-trapping ascomycete fungus, Arthrobotrys oligospora, suggesting that the lectins might also function in capturing nematodes.

• 社団法人日本農芸化学会の論文
• 2002-10-23

著者

• ANDO Akikazu

  Department of Biotechnology, Graduate School of Science and Technology, Chiba University

• NAGATA Yoshiho

  Department of Biotechnology, Graduate School of Science and Technology, Chiba University

• Yamaguchi Hiroko

  Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University

• ICHIJO NAOYA

  Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University

• IIJIMA NAOTO

  Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University

• YOSHINO Hiroto

  Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University

• TEN Lim

  Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University

• WATANABE Katsunori

  Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University

• Ichijo Naoya

  Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University

• Watanabe Katsunori

  Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University

• Ten Lim

  Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University

• Ando A

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Nagata Y

  Department Of Biotechnology University Of Tokyo

• Nagata Yuji

  Department Of Applied And Environmental Chemistry Kitami Institute Of Technology

• Iijima Naoto

  Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University

• Ando Akikazu

  Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University

• Ando Akikazu

  Department Of Agricultural Chemistry Chiba University

• Nagata Yoshiho

  Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University

• Watanabe K

  Research Institute For Food Science Kyoto University:(present Address)department Of Agricultural Che

• Nagata Y

  Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University

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