Cloning and Expression of a Novel Murine Anti-human Fas Antibody
スポンサーリンク
概要
- 論文の詳細を見る
Agonistic anti-human Fas antibodies that can induce apoptosis are thought to have therapeutic effects for various diseases resulting from an abnormality of the Fas/FasL system. However, some anti-Fas antibodies show toxicity, and it is difficult to investigate their therapeutic and toxicological effect using animals because of their species specificity. We previously obtained a murine anti-human Fas mAb, HFE7A. HFE7A reacted with both human and murine Fas, and mitigated lymphadenopathy without any sign of hepatotoxicity in MRLgld/gld mice. It is suggested that humanized HFE7A would be a therapeutic treatment for various diseases resulting from an abnormality of the Fas/FasL system. Here we isolated the cDNAs that code for the heavy and light chains of HFE7A and identified the corresponding nucleotide sequences. The recombinant HFE7A was indistinguishable in binding and apoptosisinducing activity to that from a hybridoma cell line. These data provide essential information for the humanization and clinical application of the humanized HFE7A.
- 社団法人日本農芸化学会の論文
- 2000-09-23
著者
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SERIZAWA Nobufusa
Biomedical Research Laboratories, Sankyo Co. Ltd.
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Yonehara Shin
Institute for Virus Research, Kyoto University
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YAMAGUCHI Junko
Biomedical Research Laboratories, Sankyo Co., Ltd.
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YOSHIDA-KATO Hiroko
Biomedical Research Laboratories, Sankyo Co., Ltd.
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ICHIKAWA Kimihisa
Biomedical Research Laboratories, Sankyo Co., Ltd.
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OHSUMI Jun
Biomedical Research Laboratories, Sankyo Co., Ltd.
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Yamaguchi J
Sankyo Co. Ltd. Tokyo Jpn
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Watanabe Katsunori
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
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Ohsumi Jun
Biomedical Research Laboratories Sankyo Co. Ltd.
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Yoshida-kato Hiroko
Biomedical Research Laboratories Sankyo Co. Ltd.
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Yonehara S
Graduate School Of Biostudies And Institute For Virus Research Kyoto University
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Yonehara Shin
Institute For Virus Research Kyoto University
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WATANABE Kenji
Biomedical Research Laboratories, Sankyo Co., Ltd.
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Watanabe K
Research Institute For Food Science Kyoto University:(present Address)department Of Agricultural Che
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Yamaguchi Junko
Biomedical Research Laboratories Sankyo Co. Ltd.
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Ichikawa Kimihisa
Department Of Applied Microbiology Nippon Roche Research Center
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Ichikawa Kimihisa
Biomedical Research Laboratories Sankyo Co. Ltd.
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Serizawa N
Biomedical Research Laboratories Sankyo Co. Ltd.
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Serizawa Nobufusa
Biomedical Research Laboratories Sankyo Co. Ltd.
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Watanabe Kenji
Biomedical Research Laboratories Sankyo Co. Ltd.
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