Molecular Cloning and Expression of the Gene Encoding a Phospholipase A_1 from Aspergillus oryzae

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概要

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• Phospholipase A_1 (PLA_1) is a hydrolytic enzyme that catalyzes removal of the acyl group from position 1 of lecithin to form lysolecithin. The genomic DNA and cDNA encoding PLA_1 from Aspergillus oryzae were cloned with the mixed deoxyribonucleotide-primed polymerase chain reaction. The PLA_1 gene is composed of 1,056 bp and has four exons and three short introns (63,54,and 51 bp). The deduced amino acid sequence of PLA_1 contained the N-terminal sequence of the mature PLA_1 analyzed by Edman degradation. PLA_1 cDNA has an open reading frame of 885 bp encoding the PLA_1 precursor of 295 amino acid residues. The mature PLA_1 is composed of 269 amino acid residues, and a prepro-sequence of 26 amino acid residues is at the N-terminal region of the PLA_1 precursor. PLA_1 has two possible N-glycosylation sites (Asn^27 and Asn^55). PLA_1 has a consensus pentapeptide (-Gly-His-Ser-Xaa-Gly-), which is conserved in lipases. The amino acid sequence of PLA_1 showed 47% identity with that of monoand diacylglycerol lipase from Penicillium camembertii. The PLA_1 cDNA was expressed in Saccharomyces cerevisiae KS58-2D, indicating the cloned gene to be functional.

• 社団法人日本農芸化学会の論文
• 1999-05-23

著者

• YAO Yoshio

  Biomedical Research Laboratories, Sankyo Co. Ltd.

• SERIZAWA Nobufusa

  Biomedical Research Laboratories, Sankyo Co. Ltd.

• Yao Y

  Biomedical Research Laboratories Sankyo Co. Ltd.:(present Address)science Information Co. Ltd.

• Yao Yoshio

  Biomedical Research Laboratories Sankyo Co. Ltd.

• Koishi R

  Sankyo Co. Ltd. Tokyo

• Koishi Ryuta

  Biomedical Research Laboratories Sankyo Co. Ltd.

• Tsuji T

  Manufacturing Section 2 Hiratsuka Factory Sankyo Co. Ltd.

• WATANABE Ichiro

  Biomedical Research Laboratories, Sankyo Co., Ltd.

• TSUJI Toshiaki

  Manufacturing Section 2, Hiratsuka Factory, Sankyo Co., Ltd.

• Serizawa Nobufusa

  Biomedical Research Laboratories Sankyo Co. Ltd.

• Watanabe Ichiro

  Biomedical Research Laboratories Sankyo Co. Ltd.

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• High-Level Secretory Production of Phospholipase A_1 by Saccharomyces cerevisiae and Aspergillus oryzae
• Molecular Cloning and Expression of the Gene Encoding a Phospholipase A_1 from Aspergillus oryzae
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