High-Level Secretory Production of Phospholipase A_1 by Saccharomyces cerevisiae and Aspergillus oryzae
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概要
- 論文の詳細を見る
Phospholipase A_1 (PLA_1) is a hydrolytic enzyme that catalyzes the removal of the acyl group from position 1 of lecithin to form lysolecithin. The PLA_1 gene, which had been cloned from Aspergillus oryzae, was expressed in Saccharomyces cerevisiae and A. oryzae. Through the modification of the medium composition and the feeding conditions of substrate, the production level of PLA_1 by S. cerevisiae was increased to a level fivefold higher than that indicated in a previous report. In the case of A. oryzae, introduction of multicopies of PLA_1 expression units, and the morphological change from the pellet form to the filamentous form were effective for the enhancement of PLA_1 production. We succeeded in producing 3,500 U/ml of PLA_1 using an industrial-scale fermentor.
- 社団法人日本農芸化学会の論文
- 2001-01-23
著者
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SERIZAWA Nobufusa
Biomedical Research Laboratories, Sankyo Co. Ltd.
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Fukui F
Showa Sangyo Co. Ltd. Ibaraki Jpn
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Fukui Fumio
Fermentation Research Laboratories Sankyo Co. Ltd.
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Gomi Katsuya
Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University
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WATANABE Ichiro
Biomedical Research Laboratories, Sankyo Co., Ltd.
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Yoshikawa H
Lead Discovery Research Laboratories Sankyo Co. Ltd.
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Yoshikawa Hiroji
Lead Discovery Research Laboratories Sankyo Co. Ltd.
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Ono Chiho
Lead Discovery Research Laboratories Sankyo Co. Ltd.
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Shiba Yoichiro
Lead Discovery Research Laboratories Sankyo Co. Ltd.
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FUKUI Fumio
Lead Discovery Research Laboratories, Sankyo Co. Ltd.
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Serizawa N
Biomedical Research Laboratories Sankyo Co. Ltd.
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Serizawa Nobufusa
Biomedical Research Laboratories Sankyo Co. Ltd.
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Watanabe Ichiro
Biomedical Research Laboratories Sankyo Co. Ltd.
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