Process Development for Industrial-Scale Preparation of Carboxypeptidase Y Secreted by Saccharomyces cerevisiae
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概要
- 論文の詳細を見る
A method for industrial-scale preparation of carboxypeptidase Y (CPY), which was secreted by Saccharomyces cerevisiae KS58-2D/pCY303 into the culture broth, was developed. Because the purification process consists of a few simple unit operations including only one chromatography step, a higher CPY recovery was achieved than that in the process using disrupted baker's yeast. Approximately 100 g of purified CPY powder was constantly obtained using the final culture broth from a 500-l fermentor.
- 公益社団法人日本生物工学会の論文
- 2000-10-25
著者
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Fukui F
Showa Sangyo Co. Ltd. Ibaraki Jpn
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Fukui Fumio
Fermentation Research Laboratories Sankyo Co. Ltd.
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Yoshikawa H
Lead Discovery Research Laboratories Sankyo Co. Ltd.
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Yoshikawa Hiroji
Lead Discovery Research Laboratories Sankyo Co. Ltd.
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Shiba Yoichiro
Lead Discovery Research Laboratories Sankyo Co. Ltd.
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FUKUI Fumio
Lead Discovery Research Laboratories, Sankyo Co. Ltd.
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KANARI YOSHIYUKI
Lead Discovery Research Laboratories, Sankyo Co. Ltd.
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Kanari Yoshiyuki
Lead Discovery Research Laboratories Sankyo Co. Ltd.
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