Methylalkylketone Fermentation from Palm-Kernel Oil by Penicillium decumbens IFO 7091

概要

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To make the production of methylalkylketones (MAKs) from palm-kernel oil by practical fermentation using penicillium decumbens IFO 7091,the accumulation of MAKs was studied. THe optimal culture conditions were temperature, 28℃, and initial pH, 8.2,in the basal medium. THe MAKs accumulated were methylpentylketone, methylheptylketone, methylnonylketone, and methylundecylketone; in these MAKs, methylnonylketone is major. Under the culture conditions described above, the microorganism accumulated 2.05 g of MAKs with consumption of 5.14 g of fatty acids in 8 d of shaking-culture in 100 ml of medium with 10 g of palm-kernel oil. Approximately 47% of the constituent total fatty acids of palm-kernel oil are layric acid, it was metabolized rapidly and converted to methylnonylketone in a high yield, over 46.4% (formed mehylnonylketone/consumed lauric acid), while long chain fatty acids, such as palmitic, stearic, oleic, and linoleic acids, were hardly metabolized with little MAK accumulation. In a 5-l jar fermentor, in which 270 g of palm-kernel oil were used as substrate, 58.7 g of MAKs were obtained from 144.5 g of consumed fatty acids afte 7 d of culture.
社団法人日本生物工学会の論文
1990-08-25