Cell Death Caused by Excision of Centromeric DNA from a Chromosome in Saccharomyces cerevisiae
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概要
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If genetically modified organisms (GMOs) are spread through the natural environment, it might affect the natural environment. To help prevent the spread of GMOs, we examined whether it is possible to introduce conditional lethality by excising centromeric DNA from a chromosome by site-specific recombination in Saccharomyces cerevisiae as model organism. First, we constructed haploid cells in which excision of the centromeric DNA from chromosome IV can occur due to recombinase induced by galactose. By this excision, cell death can occur. In diploid cells, cell death can also occur by excision from both homologous chromosomes IV. Furthermore, cell death can occur in the case of chromosome V. A small number of surviving cells appeared with excision of centromeric DNA, and the diploid showed greater viability than the haploid in both chromosomes IV and V. The surviving cells appeared mainly due to deletion of a recombination target site (RS) from the chromosome.
著者
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MIYAMOTO Akihiro
Department of Applied Chemistry & Biochemistry, Kumamoto University
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Matsuzaki Hiroaki
Department Of Bioengineering Faculty Of Engineering Fukuyama University
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Hatano Takushi
Department Of Bioengineering Faculty Of Engineering Fukuyama University
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Yanamoto Toshiaki
Department Of Biotechnology Faculty Of Life Science And Biotechnology Fukuyama University
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Matsumoto Takehiro
Department Of Applied Mathematics And Physics Tottori University
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