Cloning and Molecular Analysis of cDNA Encoding a Carboxymethylcellulase of the Yeast Cryptococcus flavus

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概要

• 論文の詳細を見る

• A cDNA copy for carboxymethylcellulase (CMCase 1) of the yeast Cryptococcus flavus was cloned by screening an expression cDNA library with anti-CMCase 1 antibody. The sequence of the cDNA had an open reading frame of 1023 bp that encoded a preprotein of 341 amino acids with a molecular weight of 35,698. The putative precursor begins with a hydrophobic segment that possibly acts as a signal sequence for secretion, which is followed by a presumed prosequence and a sequence consistent with the N-terminal amino acid sequence of secreted CMCase 1. No potential N-glycosylation site was found in the sequence of putative pro-CMCase 1. Comparison of the deduced protein sequence shows that the C. flavus CMCase 1 is partially homologous to the Trichoderma reesei endoglucanase EGIII. Alignment of the cDNA copy and the chromosomal DNA showed seven putative introns of 45 to 134 bp. When introduced into E. coli, the cDNA directed the synthesis of CMCase 1 as seen by CMCase activity and Western blotting using anti-CMCase 1 antibody.

• 社団法人日本農芸化学会の論文
• 1992-08-23

著者

• HATANO TAKUSHI

  Department of Bioengineering, Faculty of Engineering, Fukuyama University

• FUKUI SAKUZO

  Department of Bioengineering, Faculty of Engineering, Fukuyama University

• MIYAKAWA Tokichi

  Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni

• NAKAMURA TARO

  Department of Bio- and Geoscience, Graduate School of Science, Osaka City University

• Mochizuki Daisuke

  Department Of Fermentation Technology Faculty Of Engineering Hiroshima University

• Fukui Sakuzo

  Department Of Bioengineering Faculty Of Engineering Fukuyama University

• Hatano Takushi

  Department Of Bioengineering Faculty Of Engineering Fukuyama University

• CUI ZHIFENG

  Department of Fermentation Technology, Faculty of Engineering, Hiroshima University

• Cui Zhifeng

  Department Of Molecular Biotechnology Faculty Of Engineering Hiroshima University

• Miyakawa T

  Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive

• MATSUNO Yasuhiko

  Department of Fermentation Technology, Faculty of Engineering, Hiroshima University

• LIU Yusen

  Department of Fermentation Technology, Faculty of Engineering, Hiroshima University

• Liu Y

  Hiroshima Univ. Higashi‐hiroshima Jpn

• Miyakawa Tokichi

  Department Of Fermentation Technology

• Matsuno Yasuhiko

  Department Of Fermentation Technology Faculty Of Engineering Hiroshima University

• Nakamura Taro

  Department Of Agricultural Chemistry Faculty Fo Agriculture Hokkaido University

• Nakamura Taro

  Department Of Fermentation Technology Faculty Of Engineering Hiroshima University

• CUI Zhifeng

  Department of Bio-Geosciences, Osaka City University

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