Involvement of Thioredoxin Peroxidase Type II (Ahp1p) of Saccharomyces cerevisiae in Mn^<2+> Homeostasis
スポンサーリンク
概要
- 論文の詳細を見る
To identify new proteins involved in Mn^<2+> homeostasis, we isolated Mn^<2+> -resistant mutants of Saccharomyces cerevisiae starting from a calcineurin-deficient, Mn^<2+> hypersensitive strain (⊿cmp1 ⊿cmp2). The mutations were found to lie in the PMR1 gene, known to encode a "Ptype" Ca^<2+> -ATPase that transports Ca^<2+> and Mn^<2+> from the cytosol to the Golgi apparatus. A second gene, AHP1,was cloned as a suppressor of the Mn^<2+> tolerance of a ⊿cmp1 ⊿cmp2 pmr1 mutant. Ahp1p was recently described as a thioredoxin peroxidase type II, an antioxidant protein with alkyl hydroperoxide defense properties in yeast. AHP1 disruption in strain W303 decreased tolerance to Mn^<2+> and H_2O_2. We found that a GFP-Ahp1p fusion construct was in the cytosol when cells were grown in glucose, and in the mitochondria when cells were grown in oleate. Based on Mn^<2+> transport data, we concluded that Ahp1p is involved in cellular Mn^<2+> homeostasis in trafficking of Mn^<2+> from cytosol to mitochondria and from cytosol for export across the plasma membrane.
- 社団法人日本農芸化学会の論文
- 1999-11-23
著者
-
Hirata D
Niigata Prefectural Institute Of Brewing
-
Hirata Dai
From The Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
-
Hirata Dai
Center For Gene Science Hiroshima University
-
Tsuchiya E
Dep. Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Univ.
-
Tsuchiya Eiko
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
-
Hirata D
Hiroshima Univ. Higashi‐hiroshima Jpn
-
FARCASANU Ileana
From the Department of Fermentation Technology, Faculty of Engineering, Hiroshima University
-
TSUCHIYA Eiko
From the Department of Fermentation Technology, Faculty of Engineering, Hiroshima University
-
MIZUTA Keiko
From the Department of Fermentation Technology, Faculty of Engineering, Hiroshima University
-
MIYAKAWA Tokichi
From the Department of Fermentation Technology, Faculty of Engineering, Hiroshima University
-
Miyakawa T
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
-
Tsuchiya E
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
-
Tsuchiya Eiko
From The Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
-
Farcasanu Ileana
From The Department Of Fermentation Technology Faculty Of Engineering Hiroshima University:(present
-
Mizuta Keiko
From The Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
関連論文
- Stable Overproduction of Isoamyl Alcohol by Saccharomyces cerevisiae with Chromosome-integrated Multicopy LEU4 Genes
- Breeding of a Useful Strain of Sake Yeast for Ginjo-Sake Brewing
- Cloning, Characterization, and Expression of cDNA Encoding a Lipase from Kurtzmanomyces sp. I-11(Biochemistry & Molecular Biology)
- Purification and Characterization of a Lipase from the Glycolipid-Producing Yeast Kurtzmanomyces sp. I-11(Biochemistry & Molecular Biology)
- Isolation of Yeast Kurtzmanomyces sp. I-11, Novel Producer of Mannosylerythritol Lipid(Microbiology & Fermentation Technology)
- Fredericamycin A Affects Mitochondrial Inheritance and Morphology in Saccharomyces cerevisiae
- Extracellular Formation of Triglycerides from Glucose by a Mutant Strain of Trichosporon
- Triglyceride-Binding Proteins in the Culture Fluid of a Triglyceride-Using Fungus Strain, SM-30
- Borrelidin Inhibits a Cyclin-dependent Kinase (CDK), Cdc28/Cln2, of Saccharomyces cerevisiae
- Overexpression and Secretion of Cellulolytic Enzymes by δ-Sequence-Mediated Multicopy Integration of Heterologous DNA Sequences into the Chromosomes of Saccharomyces cerevisiae
- Expression of the Carboxymethylcellulase Gene, CMCl, from Cryptococus flavus in Saccharomyces cerevisiae
- Nucleotide Sequence of the Secretable Acid Protease Gene PEP1 in the Yeast Saccharomycopsis fibuligera(Biological Chemistry)
- Characteristic Decrease of Nuclear Protein Kinase Activity in Gl-Arrested Cells of Saccharo-myces cerevisiae cdc28
- Cloning and Molecular Analysis of cDNA Encoding a Carboxymethylcellulase of the Yeast Cryptococcus flavus
- Purification and Characterization of a Carboxymethylcellulose-Degrading Enzyme Secreted by a Yeast Strain Newly Isolated from Soil
- The Rpd3/HDAC Complex Is Present at the URS1 cis-Element with Hyperacetylated Histone H3
- Involvement of Thioredoxin Peroxidase Type II (Ahp1p) of Saccharomyces cerevisiae in Mn^ Homeostasis
- Functional Interaction of Isr1,a Predicted Protein Kinase, with the Pkc1 Pathway in Saccharomyces cerevisiae
- The Physiological Roles of Membrane Ergosterol as Revealed by the Phenotypes of syr1/erg3 Null Mutant of Saccharomyces cerevisiae
- Functional Analysis of the Promoter of the Saccharomyces cerevisiae Multidrug Resistance Gene YDR1, which Encodes a Member of the ATP Binding Cassette (ABC) Superfamily
- A Novel Method of Screening Cell-Cycle Blockers as Candidates for Anti-Tumor Reagents Using Yeast as a Screening Tool
- Expression of Mutant RPA in Human Cancer Cells Causes Telomere Shortening
- RSC Nucleosome-remodeling Complex Plays Prominent Roles in Transcriptional Regulation throughout Budding Yeast Gametogenesis
- A Double Mutant between Fission Yeast Telomerase and RecQ Helicase Is Sensitive to Thiabendazole, an Anti-Microtubule Drug