Triglyceride-Binding Proteins in the Culture Fluid of a Triglyceride-Using Fungus Strain, SM-30
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概要
- 論文の詳細を見る
A fungus, SM-30 was isolated as using triglyceride and hardly using oleate, and identified as a member of the genus Trichoderma. In the culture fluid of the organism, the presence of triglyceride-binding proteins (TG-proteins) was demonstrated by an over-lay method; two TG-proteins with molecular weights (MWs) of 24 and 8 kDs (10^3 daltons) in the culture of soybean oil medium, while in the culture of glucose medium, only one TG-protein (MW 8 kD) was found. These proteins were purified and their biochemical and physicochemical properties were studied. All of them showed affinities to a triglyceride (triolein) and triglyceride-emulsion-stabilizing activities without any lipase activity. The comparison of the emulsion-stabilizing activities in the three culture fractions, such as extracellular (culture fluid), cell-membranous (cell envelope), and intracellular (cytoplasm) fractions, indicated that the gradient of the stabilizing activity in the culture is intracellular-high and extracellularlow. Subsequently, the entry flow of triglyceride in the fungus might be superior in speed to the exit flow.
- 社団法人日本生物工学会の論文
- 1990-08-25
著者
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Yagi Takashi
Research And Development Center Showa Sangyo Co. Ltd.
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HATANO TAKUSHI
Department of Bioengineering, Faculty of Engineering, Fukuyama University
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FUKUI SAKUZO
Department of Bioengineering, Faculty of Engineering, Fukuyama University
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Yagi T
Showa Sangyo Co. Ltd. Funabashi Jpn
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MIYAKAWA Tokichi
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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KAWAGUCHI MIEKO
Department of Bioengineering, Faculty of Engineering, Fkuyama University
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YAGI TAKASHI
Central Research Laboratory, Showa-sangyo Co., Ltd.,
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Fukui Sakuzo
Department Of Bioengineering Faculty Of Engineering Fukuyama University
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Hatano Takushi
Department Of Bioengineering Faculty Of Engineering Fukuyama University
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Kawaguchi Mieko
Department Of Bioengineering Faculty Of Engineering Fkuyama University
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Miyakawa T
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Miyakawa Tokichi
Department Of Fermentation Technology
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