Functional Interaction of Isr1,a Predicted Protein Kinase, with the Pkc1 Pathway in Saccharomyces cerevisiae
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概要
- 論文の詳細を見る
Staurosporine is a potent inhibitor of protein kinase C. To identify the genes that functionally interact with the Pkc1 pathway of the yeast Saccharomyces cerevisiae, we screened for the genes that cause induced staurosporine sensitivity when overexpressed from a galactose-inducible promoter. The novel gene ISR1 encodes a predicted protein kinase with the highest sequence similarity to mammalian Raf in the kinase domain. Drug sensitivity induced by ISR1 overexpression is specific to staurosporine. Although ISR1 disruption causes no obvious phenotype, it does exacerbate the phenotypes of a temperature-sensitive allele (stt1-1) of PKC1,but not of the mpk1 and bck1 mutants of the Mpk1 MAP kinase pathway. These results suggest that Isr1 functions in an event important for growth in a manner redundant with a Mpk1-independent branch of the Pkc1 signalling pathways.
- 社団法人日本農芸化学会の論文
- 1998-07-23
著者
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Miyahara Kohji
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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HIRATA Dai
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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Hirata D
Niigata Prefectural Institute Of Brewing
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Hirata Dai
From The Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Hirata Dai
Center For Gene Science Hiroshima University
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Hirata Dai
Department Of Fermentation Technology Hiroshima University
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MIYAKAWA Tokichi
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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Hirata D
Hiroshima Univ. Higashi‐hiroshima Jpn
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Miyakawa T
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Miyakawa Tokichi
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Miyahara Kohji
Department Of Clinical Physiology Tokyo Metropolitan Institute Of Gerontology
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Miyakawa Tokichi
Department Of Fermentation Technology
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Miyahara Kohji
Department Of Applied Life Science Sojo University
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