Purification and Characterization of a Carboxymethylcellulose-Degrading Enzyme Secreted by a Yeast Strain Newly Isolated from Soil
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概要
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Eight yeast strains(MUT-series)possessing high abilities to utilize carboxymethylcellulose(CMC)were obtained from 127 strains previously isolated from soil. A strain MUT-6 secreted a carboxymethylcellulose-degrading enzyme(CMCase)and accumulated oligosaccharides in a CMC-medium. The CMCase was purified from the culture fluid of the yeast as a single protein(MW : 34,000 daltons)in SDS-polyacrylamide gel electro-phoresis. The N-terminal amino acid sequence of the enzyme was as follows : H-Lys-Leu-Pro-Tyr-Leu-Gly-Gly-Val-Asn-Leu-Ala-Gly-Thr-Asp-Phe-Gly-Ile-Asp-Ile-Tyr-Gly. Optimal conditions for enzyme reaction were 65-70℃ for temperature and 4.3 for pH. Heat stability was remarkably high; half-life time at 98℃(pH6.0)was 30min. Products from hydrolysis of CMC by the CMCase were di-, tri-, and tetra-oligo-saccharides without mono-mer, and could be metabolized by a transformant of Saccharomyces cerevisiae with a plasmid carrying the BGL1 gene encoding cellobiase of Saccharomycopis fibuligera. Cellobiose, as well as CMC, was found to be an effective inducer for CMCase production.
- 社団法人日本生物工学会の論文
- 1991-05-25
著者
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HATANO TAKUSHI
Department of Bioengineering, Faculty of Engineering, Fukuyama University
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FUKUI SAKUZO
Department of Bioengineering, Faculty of Engineering, Fukuyama University
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Fukui Sakuzo
Department Of Bioengineering Faculty Of Engineering Fukuyama University
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Hatano Takushi
Department Of Bioengineering Faculty Of Engineering Fukuyama University
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CUI ZHIFENG
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University
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Cui Zhifeng
Department Of Molecular Biotechnology Faculty Of Engineering Hiroshima University
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Kawaguchi Mieko
Department Of Bioengineering Faculty Of Engineering Fkuyama University
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Miyakawa T
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Miyakawa Tokichi
Department Of Fermentation Technology
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KOSAKA MUTSUKO
Department of Bioengineering, Faculty of Ebngineering, Fukuyama University
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Kosaka Mutsuko
Department Of Bioengineering Faculty Of Ebngineering Fukuyama University
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CUI Zhifeng
Department of Bio-Geosciences, Osaka City University
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