Simple Monitoring System for R-Mediated Site-specific Recombination on Chromosomes in Saccharomyces cerevisiae

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概要

• 論文の詳細を見る

• A simple measuring system for the frequency of recombinant clones generated by R-protein mediated recombination was constructed. By applying the system, the frequencies of recombinant clones due to site-specific recombination between two loci on one or two different nonhomologous chromosome(s) were monitored during cultivation. Subsequently, it was found that the frequencies increase nearly in proportion to the culture period and are different among the individual two selected loci. Thus, the system is thought to be a candidate for analyzing physicochemical situations, such as folding and localization, of chromosomes in the nucleus.

• 社団法人日本農芸化学会の論文
• 1994-09-23

著者

• HATANO TAKUSHI

  Department of Bioengineering, Faculty of Engineering, Fukuyama University

• FUKUI SAKUZO

  Department of Bioengineering, Faculty of Engineering, Fukuyama University

• YAMANE Shoji

  Department of Rheumatology and Clinical Immunology, Clinical Research Center for Rheumatology and Al

• MATSUZAKI HIROAKI

  Department of Bioengineering, Faculty of Engineering, Fukuyama University

• Matsuzaki Hiroaki

  Department Of Bioengineering Faculty Of Engineering Fukuyama University

• OSHIMA YASUJI

  Department of Biotechnology, Faculty of Engineering, Osaka University

• Yamane S

  Okayama Univ. Sci. Okayama Jpn

• Fukui Sakuzo

  Department Of Bioengineering Faculty Of Engineering Fukuyama University

• Hatano Takushi

  Department Of Bioengineering Faculty Of Engineering Fukuyama University

• Oshima Yasuji

  Department Of Biotechnology Faculty Of Engineering Osaka University

• Yamane Shoji

  Department Of Biotechnology Faculty Of Engineering Fukuyama University

• Oshima Yasuji

  Department Of Biotechnology Faculty Of Engineering Kansai University

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