Construction of Host-Vector System in the Osmophilic Haploid Yeast Zygosaccharomyces rouxii

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概要

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• A host-vector system for Zygosaccharomyces rouxii was developed. Chimeric plasmids useful as the Escherichia coli-Z. rouxii shuttle vector were constructed with a DNA fragment of pBR322,a fragment of pSR1 plasmid of Z. rouxii or the ARS1 sequence of Saccharomyces cerevisiae, and a fragment of the LEU2 gene of S. Serevisiae or a DNA fragment bearing Tn601 which confers G-418 resistance as a selective marker of the plasmid. For the hosts, wild-type strains of Z. rouxii were modified to give improved transformation frequencies or to mark a leucine-auxotropic mutation which is complementable by the LEU2 DNA of S. cerevisiae. Transformation frequencies from several hundred to two thousand transformant clones per μg plasmid DNA samples were obtained.

• 社団法人日本生物工学会の論文
• 1988-10-25

著者

• Araki Hiroyuki

  Tochigi Research Laboratories, Kao Corporation

• TATSUMI Hiroki

  Research and Development Division, Kikkoman Corporation

• Ushio Kohei

  (present Address)research Laboratory Higashimaru Shoyu Co. Ltd.

• OSHIMA YASUJI

  Department of Biotechnology, Faculty of Engineering, Osaka University

• Tatsumi Hiroki

  (present Address)food Biotechnology Section Research Division Kikkoman Corporation

• Araki Hiroyuki

  Department Of Fermentation Technology Osaka University.

• Araki Hiroyuki

  Tochigi Research Laboratories Kao Corporation

• TOH-E AKIO

  (Present address)Department of Fermentation Technology, Faculty of Engineering, Hiroshima University

• Toh-e Akio

  (present Address)department Of Fermentation Technology Faculty Of Engineering Hiroshima University

• Oshima Yasuji

  Department Of Biotechnology Faculty Of Engineering Osaka University

• Oshima Yasuji

  Department Of Fermentation Technology Faculty Of Engineering Osaka University

• Araki H

  Tochigi Research Laboratories Kao Corporation

• Oshima Yasuji

  Department Of Biotechnology Faculty Of Engineering Kansai University

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