Control of Secretory Production of Human Lysozyme from Saccharomyces cerevisiar by Incubation Temperature and Phosphate Concentration
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概要
- 論文の詳細を見る
A system for the controlled expression of a foreign gene in Saccharomyces cerevisiae by temperature and/or inorganic phosphate (P_i) concentration in the medium was constructed. A DNA fragment bearing the promoter of the PHO84 gene, which encodes a P_i transporter of S. cerevisiae and is derepreassed by P_i starvation, was used as promoter. When a cDNA fragment encoding the human lysozyme (h-lysozyme) gene connected with the PHO84 promoter was ligated into a YEp vector, a maximum of 4.5mg/l of the enzyme was secreted from the host cells in low-P_i medium. When a temperature-sensitive pho81 mutant was used as the host with this vector, 2.6mg/l of h-lysozyme was secreted in low-P_i medium at 25℃ and its production was turned off at 37℃.
- 社団法人日本生物工学会の論文
- 1994-04-25
著者
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Harashima Satoshi
Department of Biotechnology, Graduate School of Engineering, Osaka University
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BUN-YA Masanori
Faculty of Integrated Arts and Sciences, Hiroshima University
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Harashima Satoshi
Department Of Biotechnology Faculty Of Engineering Osaka University
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OSHIMA YASUJI
Department of Biotechnology, Faculty of Engineering, Osaka University
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Oshima Yasuji
Department Of Biotechnology Faculty Of Engineering Osaka University
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HIRAOKA EMI
Molecular Biology Department, Nippon Shinyaku Co. Ltd.,
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MURAI MASATOSHI
Molecular Biology Department, Nippon Shinyaku Co. Ltd.,
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YANO JYUN-ICHI
Molecular Biology Department, Nippon Shinyaku Co. Ltd.,
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BUN-YA MASANORI
Department of Biotechnology, Faculty of Engineering, Osaka University
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Yano Jyun-ichi
Molecular Biology Department Nippon Shinyaku Co. Ltd.
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Bun-ya Masanori
Faculty Of Integrated Arts And Sciences Hiroshima University
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Murai Masatoshi
Molecular Biology Department Nippon Shinyaku Co. Ltd.
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Oshima Yasuji
Department Of Biotechnology Faculty Of Engineering Kansai University
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Hiraoka Emi
Molecular Biology Department Nippon Shinyaku Co. Ltd.
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HARASHIMA SATOSHI
Department of Biotechnology, Faculty of Engineering, Osaka University
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HARASHIMA Satoshi
Department of Biotechnoiogy, Graduate School of Engineering, Osaka University
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