Construction of a Saccharomyces cerevisiae strain with a high level of RNA(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
スポンサーリンク
概要
- 論文の詳細を見る
A strategy has been developed for creating Saccharomyces cerevisiae strains with a high RNA content by following a three-step breeding procedure. In the first step, an S. cerevisiae disruptant of the RRN10 gene, one of the components of the UAF (upstream activation factor) complex of rRNA transcription, was constructed and showed severely slow growth. In the second step, seven suppressors were isolated that restored the slow growth of the Δrrn10 disruptant Genetic analysis revealed that each of the seven suppressors that were isolated appeared to have dominant and multiple mutations. The specific growth rate of those suppressors was increased approximately two-fold as compared with the Δrrn10 parental strain. The absolute RNA content showed that the suppressors had an RNA content 32-56% higher than that of the Δrrn10 parental strain. In the last step, the RRN10 wild-type gene was integrated into chromosome V of each of the original suppressors. The total RNA content of the integrants was also 1.4- to 2.3-fold higher than the wild-type strain. In conclusion, since yeast RNA is the source of 5'-IMP and 5'-GMP that enhance the delicious taste in certain types of food, like soups and sauces, the strategy taken in this study provides effective approach to breed S. cerevisiae strains producing a higher content of RNA that will contribute to yeast food biotechnology.
著者
-
Sugiyama Minetaka
Department of Biotechnology, Graduate School of Engineering, Osaka University
-
Kaneko Yoshinobu
Department of Biotechnology, Graduate School of Engineering, Osaka University
-
Harashima Satoshi
Department of Biotechnology, Graduate School of Engineering, Osaka University
-
NISHIUCHI HIROAKI
Ajinomoto Co.,Inc.
-
Harashima S
Department Of Biotechnology Graduate School Of Engineering Osaka University
-
Harashima Satoshi
Department Of Biotechnology Faculty Of Engineering Osaka University
-
Kim Yeon-hee
Dept. Biotech. Grad. Sch. Eng. Osaka Univ.
-
Kaneko Y
Department Of Biotechnology Graduate School Of Engineering Osaka University
-
Kaneko Yoshinobu
Department Of Biotechnology Graduate School Of Engineering Osaka University
-
Miwa Haruhumi
Ajinomoto Co. Inc.
-
Kaneko Yoshinobu
Dept. Biotech. Grad. Sch. Eng. Osaka Univ.
-
Harashima Satoshi
Dept. Biotech. Grad. Sch. Eng. Osaka Univ.
-
Chuwattanakul Varesa
Dept. Biotech. Grad. Sch. Eng. Osaka Univ.
-
Sugiyama M
Department Of Biotechnology Graduate School Of Engineering Osaka University
-
Sugiyama Minetaka
Department Of Biochemical Engineering & Science Kyushu Institute Of Technology
-
Chuwattanakul Varesa
Department of Biotechnology, Graduate School of Engineering, Osaka University
-
Kim Yeon-Hee
Department of Biotechnology, Graduate School of Engineering, Osaka University
-
Miwa Haruhumi
Ajinomoto Co. Inc
-
Chuwattanakul Varesa
Department Of Biotechnology Graduate School Of Engineering Osaka University
-
Nishiuchi Hiroaki
Ajinomoto Co. Inc
-
Minetaka Sugiyama
Dept. Biotech. Grad. Sch. Eng. Osaka Univ.
-
KANEKO Yoshinobu
Department of Biotechnoiogy, Graduate School of Engineering, Osaka University
-
HARASHIMA Satoshi
Department of Biotechnoiogy, Graduate School of Engineering, Osaka University
関連論文
- Deciphering cellular functions of protein phosphatases by comparison of gene expression profiles in Saccharomyces cerevisiae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Development of a Host-Vector System for Lactobacillus plantarum L137 Isolated from a Traditional Fermented Food Produced in the Philippines
- Application of the Bio-Active Beads Method in Rice Transformation
- An Arabidopsis thaliana Gene on the Yeast Artificial Chromosome Can Be Transcribed in Tobacco Cells
- Molecular Cloning and Expression of Uricase Gene from Arthrobacter globiformis in Escherichia coli and Characterization of the Gene Product(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- 3P-1006 Characterization of a suppressor of Δrrn10 disruptant causing the defect in rRNA transcription in Saccharomyces cerevisiae
- 2Bp14 Molecular breeding of Saccharomyces cerevisiae strain with high amount of RNA
- A Novel Gene Delivery System in Plans with Calcium Alginate Micro-Beads
- A Novel Gene Delivery System in Plants with Calcium Alginate Micro-Beads(PLANT BIOTECHNOLOGY)
- Accumulation of Compatible Solutes, Ectoine and Hydroxyectoine, in a Moderate Halophile, Halomonas elengata KS3 Isolated from Dry Salty Land in Thailand
- Obtaining transgenic plants using the bio-active beads method
- Estimation of Cell Biomass in Plant Cell Suspensions by the Osmotic Pressure Measurement of Culture Broth
- Phylogenetic Position of Mesorhizobium huakuii subsp.rengei, a Symbiont of Astragalus sinicus cv.Japan
- 1056 Intraspesific variation of Mesorhizobium strains isolated from Astragalus sinicus cv. Japan
- Characterization of Starch-Hydrolyzing Lactic Acid Bacteria Isolated from a Fermented Fish and Rice Food, "Burong Isda", and Its Amylolytic Enzyme
- Characterization of the ATF1 and Lg-ATF1 Genes Encoding Alcohol Acetyltransferases in the Bottom Fermenting Yeast Saccharomyces pastorianus
- A Structural and Phylogenetic Study of the HO Gene from Saccharomyces bayanus var. uvarum
- Fatty Acid Desaturation in Methylotrophic Yeast Hansenula polymorpha Strain CBS 1976 and Unsaturated Fatty Acid Auxotrophic Mutants
- Effects on N-Glycosylation and Inositol on the ER Stress Response in Yeast Saccharomyces cerevisiae
- Mass Mating Method in Combination with G418- and Aureobasidin A-Resistance Markers for Efficient Selection of Hybrids from Homothallic Strains in Saccharomyces cerevisias
- Genes in PHT Plasmid Encoding the Initial Degradation Pathway of Phthalate in Pseudomonas putida
- 3P-1005 Physiological characterization of high-temperature tolerant Saccharomyces cerevisiae strain and identification of a responsible gene
- Construction and Characterization of Single-Gene Chromosomes in Saccharomyces cerevisiae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Creating a Saccharomyces cerevisiae Haploid Strain Having 21 Chromosomes(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- 1102 Cloning and functional analysis of Δ^9-fatty acid desaturase gene of Hansenula polymorpha
- Efficient Production of Chitinase by Immobilized Wasabia japonica Cells in Double-Layered Gel Fibers
- Bacterial Alginate Lyase : Properties of the Enzyme Formed in a Mixed Culture of Bacteria Isolated from Soil
- Symbiotic Formation of Alginate Lyase in Mixed Culture of Bacteria Isolated from Soil
- Use of the PDR4 Gene as a Dominant Selective Marker in Combination with Cerulenin for Prototrophic Strains in Sacchromyces cerevisiae
- A Method for Direct Selection of Mating-Competent Clones from Mating-Incompetent Industrial Strains of Saccharomyces cerevisiae
- A System for Temperature-Controlled Expression of a Foreign Gene with Dual Mode in Saccharomyces cerevisiae
- 159 Four XY females found at the time of surgery for inguinal hernia
- Improvement and Application of a Promoter-Probe Vector Bearing the PHO5 Gene as the Indicator Marker in Saccharomyces cerevisiae
- Partial Restoration of Sporulation Defect in Sake Yeasts, Kyokai No. 7 and No. 9,by Increased Dosage of the IME1 Gene
- The Transcriptional Activators of the PHO Regulon, Pho4p and Pho2p, Interact Directly with Each Other and with Components of the Basal Transcription Machinery in Saccharomyces cerevisiae^1
- 1019 Cloning and characterization of Δ 9-fatty acid desaturase genes from Pichia, Yarrowia and Kluyveromyces
- A Simple PCR Method for Distinguishing Saccharomyces cerevisiae from Its Sibling species by Amplification of the RPL2 Region
- PHT, a Transmissible Plasmid Responsible for Phthalate Utilization in Pseudomonas putida
- A Simple Method for Detection of Enzyme Activities Involved in the Initial Step of Phthalate Degradation in Microorganisms
- 556 Cloning and functional analysis of FAS1 gene encoding fatty acid synthetase in Hansenula polymorpha
- Genetic Characterization of rbt Mutants That Enhance Basal Transcription from Core Promoters in Saccharomyces cerevisiae^1
- Control of Secretory Production of Human Lysozyme from Saccharomyces cerevisiar by Incubation Temperature and Phosphate Concentration
- Identification and Functional Characterization of Yeast ζ-COP
- Saccharomyces cerevisiae protein phosphatase Ppz1 and protein kinases Sat4 and Ha15 are involved in the control of subcellular localization of Gln3 by likely regulating its phosphorylation state(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Application of the PHO5-Gene-Fusion Technology to Molecular Genetics and Biotechnology in Yeast
- A Method for Fusing Chromosomes in Saccharomyces cerevisiae
- One-Step Splitting of a Chromosome in Haploid Cells of Saccharomyces cerevisiae and Its Effect on the Cell Proliferation
- One-step splitting of a chromosome in haploid cells of Saccharomyces cerevisiae
- A Structural and Phylogenetic Study of the HO Gene from Saccharomyces bayanus var. uvarum
- Strategy for preventing bacterial contamination by adding exogenous ethanol in solid-state semi-continuous bioethanol production(BIOCHEMICAL ENGINEERING)
- Construction of a Saccharomyces cerevisiae strain with a high level of RNA(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Tetraploid Formation through the Conversion of the Mating-type Alleles by the Action of Homothallic Genes in the Diploid Cells of Saccharomyces Yeasts
- A Yeast Artificial Chromosome-Splitting Vector Designed for Precise Manipulation of Specific Plant Chromosome Region(Genetics, Molecular Biology, and Gene Engineering)
- Repeated Chromosome Splitting Targeted to δ Sequences in Saccharomyces cerevisiae
- Modification of Metabolic Pathways of Saccharomyces cerevisiae by the Expression of Lactate Dehydrogenase and Deletion of Pyruvate Decarboxylase Genes for the Lactic Acid Fermentation at Low pH Value
- Saccharomyces sensu stricto : Systematics, Genetic Diversity and Evolution
- A Rapid and Precise Method to Determine Cysteine Content in Food Materials using 4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole
- 2Hp11 Highly efficient bioethanol production by a Saccharomyces cerevisiae strain with multiple stress tolerance to high temperature, acid and ethanol
- 1Fp06 Supraoptimal temperatures tolerance of Saccharomyces cerevisiae can be achieved by over-expression of RSP5 gene encoding ubiquitin ligase
- Lactic-acid stress causes vacuolar fragmentation and impairs intracellular amino-acid homeostasis in Saccharomyces cerevisiae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Large-scale genome reorganization in Saccharomyces cerevisiae through combinatorial loss of mini-chromosomes(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Increased transcription of NOP15, involved in ribosome biogenesis in Saccharomyces cerevisiae, enhances the production yield of RNA as a source of nucleotide seasoning(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Characterization and gene expression profiles of thermotolerant Saccharomyces cerevisiae isolates from Thai fruits(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Lactic-acid stress causes vacuolar fragmentation and impairs intracellular amino-acid homeostasis in Saccharomyces cerevisiae
- Genetic interactions of ribosome maturation factors Yvh1 and Mrt4 influence mRNA decay, glycogen accumulation, and the expression of early meiotic genes in Saccharomyces cerevisiae
- An Improved Method to Determine Gamma-glutamylcysteine Content in Foodstuffs Using 4-(Aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole
- Construction and Characterization of Single-Gene Chromosomes in Saccharomyces cerevisiae
- Large-scale genome reorganization in Saccharomyces cerevisiae through combinatorial loss of mini-chromosomes
- Preparation of a γ-glutamylcysteine-enriched yeast extract from a newly developed GSH2-deficient strain(BREWING AND FOOD TECHNOLOGY)
- Novel method for screening Saccharomyces cerevisiae mutants with increased sulfur-containing compounds : Color-based selection of colonies using the met30 strain(BREWING AND FOOD TECHNOLOGY)
- Novel method for screening Saccharomyces cerevisiae mutants with increased sulfur-containing compounds : Color-based selection of ade1 or ade2 mutants(BREWING AND FOOD TECHNOLOGY)
- Enhanced bio-ethanol production from cellulosic materials by semi-simultaneous saccharification and fermentation using high temperature resistant Saccharomyces cerevisiae TJ14(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- A Combination of Flow Cytometry and Traditional Screening Using Chemicals to Isolate High Glutathione-Producing Yeast Mutants
- Increased transcription of NOP15, involved in ribosome biogenesis in Saccharomyces cerevisiae, enhances the production yield of RNA as a source of nucleotide seasoning
- Functionally redundant protein phosphatase genes PTP2 and MSG5 co-regulate the calcium signaling pathway in Saccharomyces cerevisiae upon exposure to high extracellular calcium concentration(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Disruption of multiple genes whose deletion causes lactic-acid resistance improves lactic-acid resistance and productivity in Saccharomyces cerevisiae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Characterization and gene expression profiles of thermotolerant Saccharomyces cerevisiae isolates from Thai fruits
- Increased transcription of RPL40A and RPL40B is important for the improvement of RNA production in Saccharomyces cerevisiae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Suppression mechanism of the calcium sensitivity in Saccharomyces cerevisiae ptp2Δmsg5Δ double disruptant involves a novel HOG-independent function of Ssk2, transcription factor Msn2 and the protein kinase A component Bcy1(GENETICS, MOLECULAR BIOLOGY, AND
- Novel method for screening Saccharomyces cerevisiae mutants with increased sulfur-containing compounds : Color-based selection of colonies using the met30 strain
- Novel method for screening Saccharomyces cerevisiae mutants with increased sulfur-containing compounds : Color-based selection of ade1 or ade2 mutants
- Functionally redundant protein phosphatase genes PTP2 and MSG5 co-regulate the calcium signaling pathway in Saccharomyces cerevisiae upon exposure to high extracellular calcium concentration
- Enhanced bio-ethanol production from cellulosic materials by semi-simultaneous saccharification and fermentation using high temperature resistant Saccharomyces cerevisiae TJ14