Characterization of Starch-Hydrolyzing Lactic Acid Bacteria Isolated from a Fermented Fish and Rice Food, "Burong Isda", and Its Amylolytic Enzyme
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概要
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Nine strains of lactic acid bacteria that hydrolyze starch were isolated from burong isda, and indigenous fermented food made from fish and rice in the Philippines. Conventional taxonomic and DNA-DNA reassociation studies indicated that all these isolates belong to Lactobacillus plantarum. Each of these isolates harbored more than ten plasmid species with molecular sizes of 2 to 60 kb. The amylolytic activity of L137,one of the isolates, was lost by treatment with novobiocin at 43% frequency, concomitant with curing of a 33-kb plasmid, pLTK13;this suggested that pLTK13 carries a gene necessary for synthesis of amylolytic enzyme. An acidophilic starch-hydrolyzing enzyme secreted from L137 cells was purified 46-fold with specific activity of 44 units per mg protein. The enzyme was shown to have a molecular mass of about 230 kDa and the optimum temperature and pH for the enzyme reaction with soluble starch were 35℃ and 3.8-4.0,respectively. The enzyme hydrolyzed soluble starch, amylopectin, glycogen, and pullulan, and to a small extent amylosel, while it exerted no activity on dextran and cyclodextrins. The major reaction products from soluble starch were maltotriose, maltotetraose and maltopentaose, but no panose was detected, and maltotriose was the sole product from pullulan. The K_m values for soluble starch, pullulan, and amylose were 4.0,5.1,and 33 g per liter, respectively.These observation suggest that this enzyme hydrolyzes both α-1,6 and α-1,4-glucosidic linkages.
- 社団法人日本生物工学会の論文
- 1995-08-25
著者
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Kaneko Yoshinobu
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Ono Hisayo
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Ono Hisayo
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Ono Hisayo
Department Of Fermentation Technology Osaka University
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Ono Hisayo
Department Of Biotechnology Faculty Of Engineering Osaka University
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Ono H
Manufacturing Technology Laboratories:fujisawa Pharmaceutical Co. Ltd.
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Olympia Minerva
Institute Of Fish Processing Technology College Of Fisheries University Of The Philippines In The Vi
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TAKANO MITSUO
Department of Biotechnology, Graduate School of Engineering, Osaka University
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FUKUDA HAJIME
Department fo Biotechnology, Faculty of Engineering, Osaka University
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Fukuda Hajime
Department Fo Biotechnology Faculty Of Engineering Osaka University
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Kaneko Y
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Kaneko Yoshinobu
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Takano M
Institute Of Genetic Ecology Tohoku Universtiy
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Takano Mitsuo
Department of Biotechnology, Faculty of Engineering, Osaka University
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KANEKO Yoshinobu
Department of Biotechnoiogy, Graduate School of Engineering, Osaka University
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