Molecular Analysis of Promoter Elements from Propionibacterium freudenreichii (GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
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概要
- 論文の詳細を見る
Propionibacterium freudenreichii is a commercially important microorganism that is used in the production of cheeses, cobalamin (vitamin B_<12>), and propionic acid. Although a host-vector system in propionibacteria has been developed, there is little information available on the genetic background of the bacteria. To obtain genetic information to facilitate genetic engineering in propioni-bacteria, we cloned promoter regions from P. freudenreichii using Escherichia coli as a host at the first screening and a promoter-probe vector, pCVE1, which consists of the cholesterol oxidase (choA) gene from Streptomyces sp. As a reporter gene. Finally, nine clones with strong promoter activities in P. freudenreichii were screened by monitoring the choA gene expression and determining if the nucleotide sequences of the cloned DNA fragment were aligned. The initiation sites of these transcripts were determined by primer extension analysis. The putative consensus sequences corresponding to a -35 and -10 hexamer were found to be specific for P. freudenreichii, but not E. coli or other bacteria. Moreover, a new consensus heptamerous sequence between the -35 and -10 regions, termed the -16 region, was also found. It is possible that the putative consensus heptamer is functional and essential to promoter activity in P. freudenreichii. These results should provide new opportunities for controlled gene expression in P. freudenreichii.
- 社団法人日本生物工学会の論文
- 2004-05-25
著者
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YAMASHITA MITSUO
Department of Agricultural Chemistry, Faculty of Bioresources, Mie University, Department of Biotech
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MUROOKA YOSHIKATSU
Department of Agricultural Chemistry, Faculty of Bioresources, Mie University, Department of Biotech
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Yamashita Mitsuo
Department Of Biotechnology Graduate School Of Engineering Osaka University:department Of Applied Ch
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Yamashita M
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Murooka Yoshikatsu
Department Of Biotechnology Graduate School Of Engineering Osaka University:hiroshima Institute Of T
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Murooka Y
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Ono Hisayo
Department of Biotechnology, Graduate School of Engineering, Osaka University
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PIAO YONGZHE
Department of Biotechnology, Graduate School of Engineering, Osaka University
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KAWARAICHI NAMI
Department of Biotechnology, Graduate School of Engineering, Osaka University
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ASEGAWA RYO
Department of Biotechnology, Graduate School of Engineering, Osaka University
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KIATPAPAN PORNPIMON
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Ono Hisayo
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Asegawa Ryo
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Piao Yongzhe
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Ono H
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Kawaraichi Nami
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Ono Hisayo
Department Of Biotechnology Faculty Of Engineering Osaka University
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Kiatpapan P
Department Of Biotechnology Graduate School Of Engineering Osaka University:(present Address)departm
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Kiatpapan Pompimon
Rangsit大・理・生化
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Murooka Yoshikatsu
Depart. Of Bioteclnology Graduate School Of Eng. Osaka Univ.
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Kiatpapan Pornpimon
Department Of Biotechnology Graduate School Of Engineering Osaka University:(present Address)departm
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Matsumoto Yukimasa
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Yamashita Mitsuo
Department Of Agricultural Chemistry Faculty Of Bioresources Mie University Department Of Biotechnol
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