Secretory Overproduction of Streptomyces Cholesterol Oxidase by Streptomyces lividans with a Multi-Copy Shuttle Vector
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概要
- 論文の詳細を見る
Deletion of a 1.2-kb fragment adjoined upstream of the choP-choA operon and subcloning of the operon into a multi-copy shuttle vector composed of pIJ702 and pUC19 in Streptomyces lividans resulted in the over-production of Streptomyces cholesterol oxidase extracellularyl about 70-fold more than that of the original producer, Streptomyces sp. SA-COO. When the cells of S. lividans carrying the plasmid were grown in an appropriate medium, about 90 to 99% of the enzyme produced was secreted into the medium. Increased concentration of glucose in the culture medium resulted in a significant decrease in the production of the enzyme, while elevated peptone concentrations led to a further increase in the production. The level of the overproduction of cholesterol oxidase was dependent on the copy numbers of the plasmids as well as the presence of the sequences derived from pUC19.
- 社団法人日本生物工学会の論文
- 1991-11-25
著者
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MUROOKA YOSHIKATSU
Department of Agricultural Chemistry, Faculty of Bioresources, Mie University, Department of Biotech
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Murooka Yoshikatsu
Department Of Biotechnology Graduate School Of Engineering Osaka University:hiroshima Institute Of T
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Murooka Y
Department Of Biotechnology Graduate School Of Engineering Osaka University
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HAYASHI Nobuyuki
Department of Electronic Engineering Graduate School of Engineering, Osaka University
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MOLNAR Istvan
Department of Biochemistry, Univ. of Cambridge
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CHOI KWANG-PIL
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University
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Choi Kwang-pil
Fermentation Technology Lab. Chong Kun Dang Corp.
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Molnar Istvan
Department Of Biochemistry Univ. Of Cambridge
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Molnar I
Syngenta Biotechnology Inc.
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Murooka Yoshikatsu
Depart. Of Bioteclnology Graduate School Of Eng. Osaka Univ.
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Hayashi Nobuyuki
Department Of Chemistry Faculty Of Science And Engineering Waseda University
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Hayashi Nobuyuki
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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