Gene Targeting in a Klebsiella sp. by Fusaric Acid Selection and the Use of Temperature Sensitive Replicon

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概要

• 論文の詳細を見る

• Two previously described methods for gene targeting (replacement) in Escherichia coli were applied to the disruption of the maoCa operon in Klebsiella aerogenes. These techniques involve plasmid-chromosomal integration, resolution of the integrated intermediate and segregation (i) screened using fusaric acid for the counter selection of plasmid replicons carrying the gene for tetracycline resistance, or (ii) by using a temperature sensitive replicon. Both methods were found to be effective to create the desired chromosomal mutants in a Klebsiella strains after some modifications of the orginal experimental protocols, and may serve as tools for gene targeting studies in Klebsiella and related species.

• 公益社団法人日本生物工学会の論文
• 1994-06-25

著者

• Azakami Hiroyuki

  Department of Fermentation Technology, Faculty of Engineering, Hiroshima University

• Sugino Hiroyuki

  Department Of Applied Life Science Faculty Of Engineering Sojo University

• Sugino Hiroyuki

  Department Of Bacteriology Kurume University School Of Medicine:department Of Fermentation Technolog

• Azakami Hiroyuki

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Murooka Yoshikatsu

  Depart. Of Bioteclnology Graduate School Of Eng. Osaka Univ.

• ARAI Sumio

  Department of Microbiology, Kurume University School of Medicine

• Arai Sumio

  Department Of Bacteriology Kurume University School Of Medicine

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