Reduction of Antigenicity of Cry j I, Major Allergen of Japanese Cedar Pollen, by the Attachment of Polysaccharides(Food & Nutrition Science)
スポンサーリンク
概要
- 論文の詳細を見る
An attempt was made to mask the allergenic structure of a major allergen protein, Cry j I (CJI), in Japanese cedar pollen using the Maillard-type polysaccharide conjugation. The SDS-PAGE pattern of the CJI-galactomannan conjugate prepared by the Maillard reaction showed broad bands widely distributed from 50 kDa to more than 100 kDa, suggesting the attachment of galactomannan. The competitive enzyme-linked immunosorbent assay showed that the IgE antibody in the sera of ceder pollen-sensitive patients reacted strongly with CJI, while it did not react with the CJI-galactomannan conjugate. This result suggests that the antigenicity of CJI is greatly reduced by the conjugation with galactomannan.
- 2003-11-23
著者
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SAITO AKIRA
Department of Pediatrics, Hirosaki University School of Medicine
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USUI Masakatsu
Department of Food Science and Technology, National Fisheries University
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Azakami Hiroyuki
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University
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KATO Akio
Department of Agricultural Chemistry, Yamaguchi University
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Kato A
The Institute For Bio-medical Research Teijin Limited
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Usui Masakatsu
Department Of Biological Chemistry Yamaguchi University
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TANIGUCHI Naohiro
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
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NISHIJIMA Noriaki
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
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Azakami H
Department Of Biotechnology Graduate School Of Engineering Osaka University:school Of Dentistry Toku
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Azakami Hiroyuki
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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Taniguchi Naohiro
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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Kato A
Department Of Biological Chemistry Yamaguchi University
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Saito A
Department Of Biological Chemistry Yamaguchi University
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Nishijima Noriaki
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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Kato Akio
Department Of Agricultural Chemistry Faculty Of Agriclture Yamaguchi University
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Saito Akira
Department Of Pathology Juntendo University Urayasu Hospital
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Saito Akira
Department of Biological Chemistry, Yamaguchi University
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Saito Akira
Department of Applied and Bioapplied Chemistry, Graduate School of Engineering, Osaka City University
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