Elucidation With the Protease α-Chymotrypsin of the Inhibitory Modulating Action of Endogenous Neuropeptide Y Over Sympathetic Neurotransmission in Rat Vas Deferens
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概要
- 論文の詳細を見る
The physiological role of endogenous neuropeptide Y (NPY) in sympathetic neurotransmission was examined in rat and guinea pig vas deferens (VD), using α-chymotrypsin (α-CT). NPY-like immunoreactivity was detected in the longitudinal muscle layer of VD densely in rats but sparsely in guinea pigs, and it disappeared following surgical denervation. Under blockade of the prejunctional α2-adrenergic autoinhibition, α-CT potentiated the phasic contraction in rat, but not guinea pig, VD induced by trains of transmural nerve stimulation (TNS) in a frequency-dependent manner, which was reproducible during repeated applications and not affected by pretreatment with capsaicin. In contrast, α-CT did not potentiate the twitch response or contractions induced respectively by a single pulse TNS or by direct electrical stimulation to the smooth muscle. Exogenously applied NPY suppressed the twitch response, which was cancelled by α-CT, and excitatory junction potentials, although it affected neither spontaneous junction potentials nor the direct electrical stimulation-induced contraction. These observations provided further evidence to support that NPY is released endogenously by TNS at high frequency, acting prejunctionally to suppress sympathetic neurotransmission. Thus, the protease α-CT proved itself to be a useful tool to reveal a functional role of endogenously released peptides.
- 社団法人 日本薬理学会の論文
- 2003-02-01
著者
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Goto Katsutoshi
Departments of Internal Medicine, Surgery, Pharmacology, University of Tsukuba
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SAITO AKIRA
Department of Pediatrics, Hirosaki University School of Medicine
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Goto Katsutoshi
Department Of Pharmacology Institute Of Basic Medical Sciences University Of Tsukuba
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SHIGENOBU Koki
Department of Pharmacology, Toho University School of
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Shigenobu Koki
Department Of Chemical Pharmacology Faculty Of Pharmaceutical Sciences The University Of Tokyo
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KASUYA YUTAKA
Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, University of Tokyo
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ONO Kageyoshi
Division of Xenobiotic Metabolism Disposition, National Institute of Health Sciences
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Goto Katsutoshi
Department Of Pharmacology Institute Of Basic Medical Sciences The University Of Tsukuba
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Kasuya Y
Department Of Pharmacology Hoshi University School Of Pharmaceutical Sciences
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Kasuya Yutaka
Department Of Chemical Pharmacology Faculty Of Pharmaceutical Sciences The University Of Tokyo
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Ono Kageyoshi
Division Of Xenobiotic Metabolism And Disposition National Institute Of Health Sciences
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Ono Kageyoshi
Division Of Pharmacognosy And Phytochemistry National Institute Of Health Sciences
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Saito A
Department Of Pharmacology Institute Of Basic Medical Sciences University Of Tsukuba
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Saito Akira
Department Of Pathology Juntendo University Urayasu Hospital
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Goto Katsutoshi
Department Of Ophthalmology Kawasaki Medical School
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Saito Akira
Department of Applied and Bioapplied Chemistry, Graduate School of Engineering, Osaka City University
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