Construction and Characterization of a Bacterial Artificial Chromosome Library of the Indica Rice Kasalath
スポンサーリンク
概要
- 論文の詳細を見る
We constructed a bacterial artificial chromosome (BAC) library for the indica rice variety Kasalath using a new BAC vector, pCHR8, suited for plasmid rescue, containing nine unique restriction sites in a multicloning site. This Kasalath BAC Iibrary consists of about 65,000 clones with an average insert size of 106 kb using HindIII partially digested DNA fragments. It represents approximately sixteen rice haploid genome equivalents which are sufficient for the full coverage of the rice genome with BAC clone contigs. The library was constructed from only a single ligation mixture with high transformation efficiency. The quality of this BAC Iibrary was ascertained by successfully retrieving the BAC clones for seven rice RFLP markers. We could isolate eleven individual BAC clones using RFLP markers that linked to the lethal factor locus for segregation distortion on rice chromosome 3. The content of the chloroplast and mitochondrial DNA in this library was estimated to be 2.8% and 0.4%, respectively. These results demonstrate that this Kasalath BAC Iibrary should be suitable for map-based cloning and physical mapping of the rice genome.
- 日本育種学会の論文
著者
-
SAITO AKIRA
Department of Pediatrics, Hirosaki University School of Medicine
-
Miyazaki Chikara
Nagasaki Laboratory, Japan Science and Technology Corporation
-
Miyazaki C
Kyusyu Agricultural Experimental Station Kumamoto Jpn
関連論文
- Electrocardiographic and Electrophysiological Studies on Arrhythmias Following Streptococcal Infection in School Children : Proceedings of the IV Conference on Prevention for Rheumatic Fever and Rheumatic Heart Disease, January 1979, Kyoto
- Two-dimensional Submicron Focusing of Hard X-rays by Two Elliptical Mirrors Fabricated by Plasma Chemical Vaporization Machining and Elastic Emission Machining
- Force Microscopy Study of SrTiO_3(001) Surfaces with Single Atomic-Layer Steps
- Magnetic Resonance Imaging Findings in Primary Amyloidosis-associated Arthropathy
- Comparison of Gene Delivery Methods for Transformation of Robinia pseudoacacia L.by Ti-plasmid
- The Sharing of Target-Epitopes between Human Anti-ABO Hemagglutinating and Anti-Pig (Xeno) Endothelium or Anti-Pig Thyroglobulin Antibodies
- Pituicytoma : Two Case Reports
- Axial Symptoms After Cervical Laminoplasty With C3 Laminectomy Compared With Conventional C3-C7 Laminoplasty : A Modified Laminoplasty Preserving the Semispinalis Cervicis Inserted into Axis
- -1090-IMPORTANCE OF CALCITONIN GENE-RELATED PEPTIDE (CGRP)-CONTAINING NERVES IN THE REGULATION OF HUMAN VASCULAR TONUS : THE 54th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
- Effects of a Solute Analogue p-Acetoacetanisidide on the Crystallization of p-Acetanisidide
- Aggregation of p-Acetanisidide Molecules in the Under- and Super-saturated Solution and Its Effect on Crystallization
- Left Atrial Ball Thrombi without Mitral Valve Disease treated by Surgical Removal
- Elucidation With the Protease α-Chymotrypsin of the Inhibitory Modulating Action of Endogenous Neuropeptide Y Over Sympathetic Neurotransmission in Rat Vas Deferens
- Progressive Glomerulonephritis with Increasing Proteinuria Induced by a Second Attack to the Mesangial Cell
- Selection, Regeneration and Protein Profile Characteristics of NaCl-Tolerant Callus of Robinia pseudoacacia L.
- Plantlets Regeneration from Root Explants of Robinia pseudoacacia L. by Suspension Culture
- Efficacy of Continuous Cleansing with Teicoplanin on Post-CABG Methicillin-resistant Staphylococcus Aureus (MRSA) Mediastinitis : Report of a Case
- Characterization of Sb Atomic-Layer-Doped Si(100) Crystal by X-Ray Standing Wave Method
- Reduction of Antigenicity of Cry j I, Major Allergen of Japanese Cedar Pollen, by the Attachment of Polysaccharides(Food & Nutrition Science)
- Improved Functional Properties of the Ovoinhibitor by Conjugating with Galactomannan(Food & Nutrition Science)
- Functional Properties of Glycosylated Lysozyme Secreted in Pichia pastoris(Food & Nutrition Sience)
- Magnetic Resonance Imaging of Large and Giant Intracranial Aneurysms
- Stark Effect in CH_3OH Submillimeter Lasers
- Study of the Si(111)√×√-Sb Structure by X-Ray Diffraction
- Crystal Structures of the Room-and Low-Temperature Phases of Monoclinic Potassium Ferricyanide
- Application of Simple Mechanical Polishing to Fabrication of Nanogap Flat Electrodes
- Construction of Independently Driven Double-Tip Scanning Tunneling Microscope
- Scanning Tunneling Microscopy Observation of Langmuir-Blodgett Diacetylene Compound Films Deposited by Schaefer's Method (Short Note)
- A Case of Transient Bioprosthetic Valve Regurgitation and Hemolysis Devoloping Early after Surgery Using Carpentier-Edwards Valve
- Rerepair for Recurrent Mitral Regurgitation Due to Physio Ring Dehiscence
- Structural and Functional Properties of Chicken Lysozyme Fused Serine-rich Heptapeptides at the C-Terminus
- Secretion of Glycosylated α-Lactalbumin in Yeast Pichia pastoris
- Effects of Calnexin Deletion in Saccharomyces cerevisiae on the Secretion of Glycosylated Lysozymes
- IB-29 Histochemical study on the formation of cell polarity in a human colon cancer cell line HT-29, with special reference to cytoskeleton and cell-cell junction molecules(THE 46TH ANNUAL MEETING OF THE JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY)
- II-B-19 Localization of γ-tubulin-like molecule in the mouse duodenal epithelium(THE 45TH ANNUAL MEETING OF THE JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY)
- Relationship between the Stability of Hen Egg-White Lysozymes Mutated at Sites Designed to Interact with α-Helix Dipoles and Their Secretion Amounts in Yeast
- RELATIONSHIP BETWEEN LEFT VENTRICULAR RELAXATION PERIOD AND APPEARANCE OF HYPERTROPHY IN ESSENTIAL HYPERTENSION : Hemodynamics, Cardiac Function (IV) : IV : 48 Annual Scientific Meeting, Japanese Circulation Society
- Living Ring-Opening Metathesis Polymerization of Exo-Norbornenes Bearing Both Cyano and Ester Functionalities by a Well-Defined Ruthenium Catalyst
- Acute Acalculous Cholecystitis After Cardiovascular Surgery
- Prevalence of Human Papillomavirus Infection and Its Correlation with Cervical Lesions in Commercial-Sex Workers in Japan
- Intramedullary Subependymoma with Neurofibromatosis : Report of Two Cases
- Relationship between the Stability of Hen Egg-White Lysozymes Mutated at Sites Designed to Interact with α-Helix Dipoles and Their Secretion Amounts in Yeast
- Sarcomatoid carcinoma and carcinosarcoma of the urinary bladder
- DEPLETION OF CALCITONIN GENE-RELATED PEPTIDE (CGRP) BY CAPSAICIN IN CEREBRAL ARTERIES
- A COMPARATIVE STUDY OF CHANGES IN INNERVATION AND DEVELOPMENT OF SUPERSENSITIVITY IN THE RAT VAS DEFERENS AFTER VARIOUS PROCEDURES
- A Convergent, Homochiral Bis (amino alcohol) for Obtaining High Enantioselectivity in the Addition of Diethylzinc to Aldehydes
- イネペルオキシダ-ゼ遺伝子のRFLPマッピング--アイソザイム分析によるものとは異なる新しい1遺伝子座の検出〔英文〕
- Alpha-1 Antitrypsin Deficiency with Severe Pulmonary Emphysema
- Prediction of metastases in melanoma patients with positive sentinel node : Histological and molecular approach
- Preparation of Gas of Constant Humidity by a By-pass Humidifier Technique
- Mapping of Quantitative Trait Loci Conferring Resistance to Barley Yellow Mosaic Virus in a Chinese Barley Landrace Mokusekko 3
- A Barly Linkage Map Using an F_2 Population Compared with a Map Based on Female Recombination-derived Doubled Haploid Lines
- Decomposition of Organic Substance by Activated Sludge Using Peroxide
- Nitrogen Removal by Reaction of Amine Groups with Hypochlorite
- Treatment of Dye Waste by Electrolysis Using Porous Sheet Diaphragm
- Studies on Micropropagation of Quercus acutissima Carruth
- Construction and Characterization of a Bacterial Artificial Chromosome Library of the Indica Rice Kasalath