Plantlets Regeneration from Root Explants of Robinia pseudoacacia L. by Suspension Culture
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概要
- 論文の詳細を見る
A rapid and efficient method for the regeneration of plantlets from root explants of Robinia pseudoacacia L. by suspension culture was established. The roots taken from aseptically grown 15-day-old seedlings were used as explants. It was determined that photoperiodicity was necessary for root proliferation, and that the promotive effect of IAA (3-indoleacetic acid) on root proliferation was better than that of IBA (3-indolebutyric acid). The roots cultured in l/2 MS liquid medium containing -3 μM IAA and 1% sucrose at 25℃ under 16-hour photoperiod with 50 μmol m^<-2s-1> PPFD (photosynthetic photon flux density) shaking at 100 times/min reciprocally showed high efficiency for root proliferation. BAP (6-benzylaminopurine) was found to be essential to induce adventitious shoots from the roots, and the roots cultured in the medium supplied with 3 μM BAP combined with 1 -6 μMIAA for 3 weeks under the same conditions as in the root proliferation period were most suitable for adventitious shoot inducement.
- 日本森林学会の論文
- 1998-08-16
著者
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SAITO AKIRA
Department of Pediatrics, Hirosaki University School of Medicine
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CHEN Ren
Department of Forest and Forest Products Sciences, Faculty of Agriculture, Kyushu University
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GYOKUSEN Koichiro
Department of Forest and Forest Products Sciences, Faculty of Agriculture, Kyushu University
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Gyokusen K
Kyushu Univ. Fukuoka Jpn
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Gyokusen Koichiro
Department Of Forest And Forest Products Sciences Faculty Of Agriculture Kyushu University
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Gyokusen Koichiro
Department Of Forestry Faculty Of Agriculture Kyushu University
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Chen R
Department Of Forestry Faculty Of Agriculture Kyushu University
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Chen Ren
Department Of Forest And Forest Products Sciences Faculty Of Agriculture Kyushu University
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Saito Akira
Department Of Pathology Juntendo University Urayasu Hospital
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Saito Akira
Department of Biological Chemistry, Yamaguchi University
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