Structural and Functional Properties of Chicken Lysozyme Fused Serine-rich Heptapeptides at the C-Terminus

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概要

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• Two serine-rich heptapeptides, Ser-Ser-Ser-Lys-Ser-Ser-Ser (S6K) and Ser-Ser-Ser-Ser-Ser-Ser-Ser (S7), were fused to the C-terminus of chicken lysozyme (Lz) by genetic modification to improve the functional properties of lysozyme. The cDNAs of S6K-lysozyme (S6K-Lz) and S7-lysozyme (S7-Lz) were inserted into the expression vector of Pichia pastoris and secreted in yeast cultivation medium. The secretion amounts of S6K-Lz and S7-Lz were about 60% of that of wild-type lysozyme (Wt-Lz). The CD spectra showed that the conformation of S6K-Lz and S7-Lz was conserved regardless of the attachment of serine-rich peptides. The denaturation curves of S6K-Lz and S7-Lz also showed that the conformational changes were very small. The lytic activity of S6K-Lz and S7-Lz was almost the same as that of Wt-Lz, while the bactericidal activity against Escherichia coli of S6K-Lz and S7-Lz was greatly increased. The acetic acid-urea PAGE of phosphatase-treated S6K-Lz and S7-Lz indicated the possibility of phosphorylation of the fused serine-rich heptapeptides.

• 社団法人 日本農芸化学会の論文
• 2004-06-23

著者

• SAITO AKIRA

  Department of Pediatrics, Hirosaki University School of Medicine

• Azakami Hiroyuki

  Department of Fermentation Technology, Faculty of Engineering, Hiroshima University

• KATO Akio

  Department of Agricultural Chemistry, Yamaguchi University

• Kato A

  The Institute For Bio-medical Research Teijin Limited

• XU Xiaohua

  Department of Biological Chemistry, Yamaguchi University

• KASHIMA Orie

  Department of Biological Chemistry, Yamaguchi University

• Azakami H

  Department Of Biotechnology Graduate School Of Engineering Osaka University:school Of Dentistry Toku

• Azakami Hiroyuki

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Kato A

  Department Of Biological Chemistry Yamaguchi University

• Saito A

  Department Of Biological Chemistry Yamaguchi University

• Kashima Orie

  Department Of Biological Chemistry Yamaguchi University

• Xu Xiaohua

  Department Of Biological Chemistry Yamaguchi University

• Xu Xiaohua

  Department Of Anatomy Histology And Embryology Institute Of Basic Medical Sciences Chinese Academy O

• Kato Akio

  Department Of Agricultural Chemistry Faculty Of Agriclture Yamaguchi University

• Saito Akira

  Department Of Pathology Juntendo University Urayasu Hospital

• Saito Akira

  Department of Biological Chemistry, Yamaguchi University

• Saito Akira

  Department of Applied and Bioapplied Chemistry, Graduate School of Engineering, Osaka City University

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