Amyloid Fibril Formation of Hen Lysozyme Depends on the Instability of the C-Helix (88-99)
スポンサーリンク
概要
- 論文の詳細を見る
Stable and unstable mutant lysozymes in long helices B and C were constructed to evaluate the effect of the helices on amyloid fibril formation at pH 2. Stable mutant N27D and unstable mutant K33D in the B-helix did not change in amyloid fibril formation. In contrast, stable mutant N93D and unstable mutant K97D in the C-helix showed big differences in behavior as to amyloid fibril formation. Stable mutant N93D showed a longer lag phase of aggregation and suppressed the amyloid fibril formation, whereas unstable mutant K97D showed a shorter lag phase of aggregation and accelerated amyloid fibril formation. These results suggest that the long C-helix is involved mainly in the α-helix to β-sheet transition during amyloid formation of lysozyme.
- 社団法人 日本農芸化学会の論文
- 2008-06-23
著者
-
Kato A
The Institute For Bio-medical Research Teijin Limited
-
HARADA Akihito
Department of Biological Chemistry, Yamaguchi University
-
Azakami H
Department Of Biotechnology Graduate School Of Engineering Osaka University:school Of Dentistry Toku
-
Azakami Hiroyuki
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
-
Harada Akihito
Department Of Biological Chemistry Yamaguchi University
-
Kato A
Department Of Biological Chemistry Yamaguchi University
-
Kato Akio
Department Of Agricultural Chemistry Faculty Of Agriclture Yamaguchi University
関連論文
- Purification, Characterization, and Crystallization of Monoamine Oxidase from Escherichia coli K-12
- Nucleotide Sequence of the Gene for Monoamine Oxidase (maoA) from Escherichia coli
- Conformational Changes and Functional Properties of Acid-modified Soy Protein
- Changes in the Emulsifying and Foaming Properties of Proteins during Heat Denaturation
- Determination of Molecular Weight of Soluble Ovalbumin Aggregates during Heat Denaturation Using Low Angle Laser Light Scattering Technique
- Characterization of Autoinducer 2 Signal in Eikenella corrodens and Its Role in Biofilm Formation(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Involvement of N-Acetyl-D-galactosamine-specific Lectin in Biofilm Formation by the Periodontopathogenic Bacterium, Eikenella corrodens
- Preparation of honeycomb ceramics with fine channels by fiber combustion method
- Characterization of a Novel Human Tumor Necrosis Factor-α Mutant with Increased Cytotoxic Activity
- Interactions between Novel Tumor Necrosis Factor-α Mutants and Receptors on Tumor and Normal Cells(Biological Chemistry)
- Reduction of Antigenicity of Cry j I, Major Allergen of Japanese Cedar Pollen, by the Attachment of Polysaccharides(Food & Nutrition Science)
- Improved Functional Properties of the Ovoinhibitor by Conjugating with Galactomannan(Food & Nutrition Science)
- Functional Casein-Polysaccharide Conjugates Prepared by Controlled Dry Heating
- Functional Properties of Glycosylated Lysozyme Secreted in Pichia pastoris(Food & Nutrition Sience)
- The Monoamine Regulon Including Syntheses of Arylsulfatase and Monoamine Oxidase in Bacteria
- Bactericidal Action of Lysozyme against Gram-negative Bacteria Due to Insertion of a Hydrophobic Pentapeptide into Its C-Terminus
- Ovomucin-Food Protein Conjugates Prepared through the Transglutaminase Reaction(Food & Nutrition)
- Pilot Model Using Neural Networks
- Dynamic Stability Characteristics of Aircraft and Dynamics of Pilot
- Deamidation of Ovalbumin during s-Ovalbumin Conversion
- Functional Properties of Deamidated Gluten Obtained by Treating with Chymotrypsin at Alkali pH(Food & Nutrition)
- Amyloid Fibril Formation of Hen Lysozyme Depends on the Instability of the C-Helix (88-99)
- Characterization of Recombinant Amyloidogenic Chicken Cystatin Mutant I66Q Expressed in Yeast
- Structural and Functional Properties of Chicken Lysozyme Fused Serine-rich Heptapeptides at the C-Terminus
- Expression and Characterization of Saccharomyces cerevisiae Cne1p, a Calnexin Homologue
- Secretion of Glycosylated α-Lactalbumin in Yeast Pichia pastoris
- Effects of Calnexin Deletion in Saccharomyces cerevisiae on the Secretion of Glycosylated Lysozymes
- Enantioselective Oxidation of Racemic Citronellol with an Interface Bioreactor
- Production of (S)-Citronellic Acid and (R)-Citronellol with an Interface Bioreactor
- Production of Aliphatic Carboxylic Acids via Microbial Oxidation of 1-Alkanols with Interface Bioreactor
- Improvement of the Surface Functional Properties of β-Lactoglobulin and α-Lactalbumin by Heating in a Dry State
- Relationship between the Stability of Hen Egg-White Lysozymes Mutated at Sites Designed to Interact with α-Helix Dipoles and Their Secretion Amounts in Yeast
- Effects of Freeze-denaturation of Ovomacroglobulin on the Chymosin Inhibition(Food & Nutrition)
- Emulsifying Properties of Protein-Polysaccharide Complexes and Hybrids(Food & Nutrition)
- Inhibition by Ovomacroglobulin of Pepsin and Rennin(Biological Chemistry)
- Effects of Phosphate Residues on the Excellent Emulsifying Properties of Phosphoglycoprotein Phosvitin(Food & Nutrition)
- Improvement of Gelling Properties of Ovalbumin by Heating in Dry State(Food & Nutrition)
- The Inhibitory Effects of Catechins on Biofilm Formation by the Periodontopathogenic Bacterium, Eikenella corrodens
- Relationship between the Stability of Hen Egg-White Lysozymes Mutated at Sites Designed to Interact with α-Helix Dipoles and Their Secretion Amounts in Yeast
- Gene Targeting in a Klebsiella sp. by Fusaric Acid Selection and the Use of Temperature Sensitive Replicon
- Functional Protein-Polysaccharide Conjugate Prepared by Controlled Dry-heating of Ovalbumin-Dextran Mixtures(Food & Nutrition)
- Slot Assignment Method for CDMA/NC-PRMA Systems in Multi-Cell Environments(Special Section on Multi-dimensional Mobile Information Networks)
- Genomic Recombination through Plasmid-Encoded Recombinase Enhances Hemolytic Activity and Adherence to Epithelial Cells in the Periodontopathogenic Bacterium Eikenella corrodens
- Aircraft Attitude Control by Fuzzy Control
- Singularities in the X-Ray Absorption of Metals
- On the Magnetic Susceptibility Due to the Anisotropic s-d Exchange Interaction
- Deamidation of Lysozyme during the Storage of Egg White(Food & Nutrition)
- Activation of Macrophages by Sulfated Glycopeptides in Ovomucin, Yolk Membrane, and Chalazae in Chicken Eggs
- Amyloid Fibril Formation of Hen Lysozyme Depends on the Instability of the C-Helix (88-99)
- Structural and Functional Properties of Hen Egg-white Lysozyme Deamidated by Protein Engineering
- Industrial Applications of Maillard-Type Protein-Polysaccharide Conjugates
- Development of a Simple Impulse Turbine for Nano Hydropower
- Control Law for Automatic Landing Using Fuzzy-Logic Control
- Control Augmentation Using Fuzzy Logic Control
- Cystic renal cell carcinoma diagnosed by cystofiberscopy
- LuxS affects biofilm maturation and detachment of the periodontopathogenic bacterium Eikenella corrodens(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- The Catalytic Dehydrohalogenation of Haloethanes on Solid Acids and Bases
- Elimination Reactions of Ethylene Halohydrins on Solid Catalysts
- Structure of the Vanadium-Potassium Catalyst during the Oxidation of Sulfur Dioxide and the Oxidation Mechanism
- Transition Metal Ions on Molecular Sieves. I. Complexes of Copper(II) on Y Molecular Sieves and Their Catalytic Activities
- Protein Flexibility and Functional Properties of Heat-denatured Ovalbumin and lysozyme
- Solid-state reactions between CVD-TiO2 and BaCO3.
- Functional and Structural Properties of Ovomucin
- Elimination Reactions of Propylene Chlorohydrins over Solid Acids
- The Dehalogenation of Haloalkanes on SiO2-supported Metals
- The Periodontopathogenic Bacterium Eikenella corrodens Produces an Autoinducer-2-Inactivating Enzyme
- Contribution of Structural Reversibility to the Heat Stability of the Tropomyosin Shrimp Allergen