Nucleotide Sequence of the Gene for Monoamine Oxidase (maoA) from Escherichia coli

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概要

• 論文の詳細を見る

• We found that the structural gene for monoamine oxidase was located at 30.9 min on the Escgeruchia coli chromosome. Deletion analysis showed that two amine oxidase genes are located in this region. The nueclotide sequence of one of the two genes was determined. The peptide sequence of the first 40 amino acids from the N terminus of monoamine oxidase purified from E. coli. agress with that from the nucleotide sequence of the gene. The leader peptide extends over 30 amino acids. The nucleotide sequence of the gene and amino acid sequence of the predicted mature enzyme (M.W. 81,295) were highly homologous to those of the mao A_K gene and monoamine oxidase from Klewbsiela artogenes, respectively. From these results and analysis of the enzyme activity, we concluded that the gene encodes for monamine oxidase (maoA_E). The tyrosyl residue, which may be converted to topa quinone in the E. coli ewnzyme, was located by comparison with amino acid sequences at the cofactor sites in other copper/topa quinone-containing amine oxidases.

• 社団法人日本生物工学会の論文
• 1994-03-25

著者

• YAMASHITA MITSUO

  Department of Agricultural Chemistry, Faculty of Bioresources, Mie University, Department of Biotech

• MUROOKA YOSHIKATSU

  Department of Agricultural Chemistry, Faculty of Bioresources, Mie University, Department of Biotech

• Yamashita Mitsuo

  Department Of Biotechnology Graduate School Of Engineering Osaka University:department Of Applied Ch

• Yamashita M

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Murooka Yoshikatsu

  Department Of Biotechnology Graduate School Of Engineering Osaka University:hiroshima Institute Of T

• Murooka Y

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Kumagai H

  Kyoto Univ. Kyoto Jpn

• KUMAGAI HIDEHIKO

  Department of Food Science and Technology, Kyoto University

• SUZUKI Hideyuki

  Department of Food Science and Technology, Faculty of Agriculture, Kyoto University

• Kumagai H

  Kyoto Univ.

• Kumagai H

  Nihon Univ. Fujisawa‐shi Jpn

• Kumagai Hidehiko

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University

• Roh Jung

  Department of Food Science and Technology, Faculty of Agriculture, Kyoto University

• Azakami Hiroyuki

  Department of Fermentation Technology, Faculty of Engineering, Hiroshima University

• ROH JUNG-HYEOB

  Department of Food Science and Technology, Faculty of Agriculture, Kyoto University

• Roh Jung

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University

• Kumagai Hidehiko

  Division Of Integrated Life Sciences Graduate School Of Biostudies Kyoto University

• Suzuki H

  Kyoto Univ. Kyoto Jpn

• Suzuki H

  The United Graduate School Of Agricultural Science Gifu University

• Azakami H

  Department Of Biotechnology Graduate School Of Engineering Osaka University:school Of Dentistry Toku

• Azakami Hiroyuki

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Murooka Yoshikatsu

  Depart. Of Bioteclnology Graduate School Of Eng. Osaka Univ.

• Suzuki Hideyuki

  Department Of Environmental And Ocean Engineering University Of Tokyo

• Yamashita Mitsuo

  Department Of Agricultural Chemistry Faculty Of Bioresources Mie University Department Of Biotechnol

• Suzuki Hideyuki

  Department Of Biological Science And Technology Science University Of Tokyo

• Suzuki Hideyuki

  Department Of Bio-medical Engineering School Of High-technology For Human Welfare Tokai University

• KUMAGAI Hidehiko

  Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University

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