Transglycosylation Activity of the Endo-β-1, 4-Glucanase from Aspergillus niger IFO31125 and Its Application

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概要

• 論文の詳細を見る

• Endo-β-1, 4-glucanase from Aspergillus niger was found to have an endo-type transglycosylation activity. The enzyme effectively transferred cellooligosaccharide residues to various 1-alkanols in the presence of cellopentaose as the oligosaccharide donor. By incubating the enzyme with 1-octanol and cellopentaose in the presence of acetonitrile, 1-octyl-cellotrioside was synthesized. The product was isolated by silica gel column chromatography and analyzed by mass spectrometry. p-NP-β-Glc, p-NP-β-Gal, p-NP-β-GlcNAc, Ser, and Thr were also possible acceptors for the transglycosylation activity of the enzyme.

• 社団法人日本生物工学会の論文
• 1999-05-25

著者

• Kumagai H

  Kyoto Univ. Kyoto Jpn

• Kumagai Hidehiko

  Research Institute For Bioresources And Biotechnology Ishikawa Prefectural University

• Kumagai Hidehiko

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Yamamoto Kenji

  Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University

• Yamamoto K

  Graduate School Of Biostudies Kyoto University

• AKIBA SHUNICHI

  Biological Science Laboratories, Kao Corporation

• Akiba Shunichi

  Biological Science Laboratories Kao Corporation

• Kumagai H

  Kyoto Univ.

• Kumagai H

  Nihon Univ. Fujisawa‐shi Jpn

• Kumagai Hidehiko

  Faculty Of Agriculture Kyoto University

• Kumagai Hidehiko

  Res. Inst. For Bioresources And Biotechnology Ishikawa Prefectural Univ.

• Kumagai Hidehiko

  Division Of Integrated Life Sciences Graduate School Of Biostudies Kyoto University

• 河合 弘康

  関西女子短期大学

• Kumagai H

  Department Of Agricultural Chemistry The University Of Tokyo

• Kumagai Hidehiko

  Graduate School Of Biostudies Kyoto University

• Yamamoto Kenji

  Division Of Integrated Life Science Graduate School Of Biostudies Kyoto University

• Yokozeki Kenzo

  Research Institute For Bioresources And Biotechnology Ishikawa Prefectural University

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