Improvement of Reticuline Productivity from Dopamine by Using Engineered Escherichia coli

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概要

• 論文の詳細を見る

• Benzylisoquinoline alkaloids (BIAs) are pharmaceutically important compounds. We have previously devised a reticuline (BIA) production method from dopamine by using Escherichia coli; however, its productivity was relatively low (33 µM, 11 mg/L). We report here, by fine-tuning the method, higher reticuline productivity of 165 µM (54 mg/L), increasing the conversion efficiency by 8-fold. These results are important for developing an efficient route to fermentative reticuline production.

• 公益社団法人 日本農芸化学会の論文

著者

• Katayama Takane

  Research Institute for Bioresource and Biotechnology, Ishikawa Prefectural University

• KUMAGAI Hidehiko

  Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University

• Kumagai Hidehiko

  Research Institute For Bioresources And Biotechnology Ishikawa Prefectural University

• Koyanagi Takashi

  Department Of Gynecology And Obstetrics Faculty Of Medicine Kyushu University

• Sato Fumihiko

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Minami Hiromichi

  Research Institute For Bioresources And Biotechnology Ishikawa Prefectural University

• Nakagawa Akira

  Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University

• KIM Ju-Sung

  Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University

• YAMAZAKI Yuya

  Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University

• MATSUMURA Eitaro

  Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University

• KOYANAGI Takashi

  Department of Food Science, Ishikawa Prefectural University

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