In Vivo Bioconversion of Tetrahydroisoquinoline by Recombinant Coclaurine N-Methyltransferase
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概要
- 論文の詳細を見る
Coclaurine N-methyltransferase from Coptis japonica catalyzes the N-methylation of coclaurine as well as simple tetrahydroisoquinoline. We examined the possibility of converting 6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline into its N-methylated product using transgenic Escherichia coli, which expressed recombinant coclaurine N-methyltransferase, without the addition of a methyl-group donor. Transgenic E. coli successfully N-methylated the substrate added to the medium and excreted the product. Limitation of bioconversion by the supply of methyl-group donor is discussed.
- 社団法人 日本農芸化学会の論文
- 2004-04-23
著者
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Sato Fumihiko
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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MORISHIGE Takashi
Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University
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Sato F
Division Of Integrated Life Science Graduate School Of Biostudies Kyoto University
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CHOI Kum-Boo
Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University
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Choi Kum-boo
Division Of Integrated Life Science Graduate School Of Biostudies Kyoto University
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Morishige Takashi
Laboratory Of Molecular And Cellular Biology Of Totipotency Division Of Integrated Life Science Grad
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Sato Fumihiko
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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