Photosynthetic Characteristics of Photoautotrophically Cultured Cells of Tobacco
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概要
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The photosynthetic characteristics of photoautotrophically cultured cells of tobacco (Nico-tiana tabacum cv. Samsun NN) as well as those of photomixotrophically cultured cells and green leaves were investigated. Analyses revealed that on a fresh weight basis cultured tobacco cells had lower chlorophyll contents than cells of green leaves. The chlorophyll content per chloroplast, however, was almost the same in both types of cell, and the chloroplast number per cell accounted for only small differences in the cellular chlorophyll content. This indicates that the larger cell volume of cultured cells is the main factor in the difference in the chlorophyll content of these cells. Photosynthetic activity measurements also showed differences in the chloroplasts of cultured and leaf cells. The maximum activities of photosystem I and the Hill reaction for the cultured cells were about half those for leaf cells on a per unit chlorophyll basis. Moreover, photoautotrophic cells had relatively constant photosystem I and Hill reaction activities during growth; whereas, on a fresh weight basis these activities in leaf cells reflected developmental changes in the chlorophyll content. Lithium dodecyl sulfate-polyacrylamide gel electrophoresis showed qualitatively similar thylakoid polypeptide compositions for cultured and leaf cells at all stages of growth even though there were quantitative decreases in the contents of several polypeptides in the cultured green cells (especially in photomixotrophic cells) in comparison to the polypeptide contents of tobacco leaves. We speculate that the lower photosynthetic activity of the cultured cells may be caused by this reduction in the contents of certain thylakoid polypeptides.
- 日本植物生理学会の論文
著者
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Sato F
Kyoto Univ. Kyoto Jpn
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Sato Fumihiko
Div. Integ. Life Sci. Grad. Sch. Biostudies Kyoto Univ.:plant Function Lab.
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Takeda S
Department Of Natural Science Osaka Women's University
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Takeda Satomi
Research Centerfor Cell and Tissue Culture, Faculty of Agriculture, Kyoto University
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Sato Fumihiko
Research Centerfor Cell and Tissue Culture, Faculty of Agriculture, Kyoto University
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Yamada Yasuyuki
Research Centerfor Cell and Tissue Culture, Faculty of Agriculture, Kyoto University
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Yamada Yasuyuki
Grad. School Of Biol. Sci. Naist
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Yamada Yasuyuki
Graduate School Of Biological Science Nara Institute Of Science And Technoiogy
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Sato Fumihiko
Research Center For Cell And Tissue Culture Faculty Of Agriculture Kyoto University
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Sato F
Division Of Integrated Life Science Graduate School Of Biostudies Kyoto University
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Yamada Y
Present Address: Graduate School Of Biological Science Nara Institute Of Science And Technology
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Sato F
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Yamada Yasuyuki
Research Center For Cell And Tissue Culture Faculty Of Agriculture Kyoto University
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YAMADA Yasuyuki
Research Center for Ceil and Tissue Culture, Faculty of Agriculture, Kyoto University
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