Effects of Physical Parameters upon Protoplast Electrofusion
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概要
- 論文の詳細を見る
The effects of three physical parameters upon protoplast electrofusion were studied using protoplasts from cultured cells of Coptis japonica and Euphorbia millii. The osmotic potential of the medium did not appreciably affect the AC-field-induced protoplast-pair formation, but significantly influenced the fusion process of the paired protoplasts in response to DC pulses. The optimum osmotic potential was 0.55 to 0.60 Osm/kg H_2O in our system. The density of the medium markedly influenced both pair formation and fusion process. The optimum density was 1.13 to 1.14 g/cm^3, and at this density the yield of the fused protoplasts increased to more than twice that of the control (1.10 g/cm^3). Hydrophilic coating of the bottom surface of the chamber with Gellan gum or polyacrylamide gel was also effective for both pair formation and the fusion process, while coating with hydrophobic silicone was entirely inhibitory. Possible interpretations of the effects of these physical parameters upon protoplast electrofusion are presented.
- 日本植物生理学会の論文
著者
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Yamada Yasuyuki
Research Centerfor Cell and Tissue Culture, Faculty of Agriculture, Kyoto University
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Morikawa Hiromichi
Research Center For Cell And Tissue Culture Faculty Of Agriculture Kyoto University
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Asada Masanori
Technical Department Nagaoka And Co.ltd.
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Morikawa H
Hiroshima Univ. Higashi‐hiroshima Jpn
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Asada Masanori
Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University
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Yamada Yasuyuki
Research Center For Cell And Tissue Culture Faculty Of Agriculture Kyoto University
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YAMADA Yasuyuki
Research Center for Ceil and Tissue Culture, Faculty of Agriculture, Kyoto University
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