Anthocyanin Production in Suspension Cultures of High-producing Cells of Euphorbia millii
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概要
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We investigated the optimal conditions for anthocyanin production in suspension cultures of a Euphorbia millii cell strain obtained by successive cell-aggregate selections. A modified Gamborgs solution containing 5 to 7% sucrose (w/v), a 1:16 ratio of NH4+:NO3-, 30 mM of nitrogen source, 10-6 M 2, 4-D, 10-8 M BA, and low concentrations of Fe2+ and SO2-4 greatly increased anthocyanin production. 2, 4-D was superior to NAA in increasing anthocyanin production. In the anthocyanin production medium we established, E. millii cells produced 32 mg anthocyanin per liter per day.
- 社団法人 日本農芸化学会の論文
著者
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Yamada Yasuyuki
Research Centerfor Cell and Tissue Culture, Faculty of Agriculture, Kyoto University
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Yamamoto Y
Department Of Biological Production Faculty Of Bioresource Sciences Akita Prefectural University
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YAMAMOTO Yoshikazu
Technical Center, Nippon Paint Co., Ltd.
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KINOSHITA Yasuhiro
Technical Center, Nippon Paint Co., Ltd.
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WATANABE Satomi
Technical Center, Nippon Paint Co., Ltd.
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YAMADA Yasuyuki
Research Center for Ceil and Tissue Culture, Faculty of Agriculture, Kyoto University
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