Extrachromosomal Circular DNA from Nuclear Fraction of Higher Plants
スポンサーリンク
概要
- 論文の詳細を見る
To determine the cellular location of extrachromosomal circular DNA in higher plants, wheat (Triticum aestivum) and tobacco (Nicotiana tabacum) nuclei were isolated and purified, and their circular DNAs were examined by electron microscopy. Covalently closed circular (ccc) DNAs were found in nuclear fractions from both species. They showed a heterogeneous size distribution ranging from 0.1 μm to more than 5 μm in contour length, with a mean of 1.7 μm (5.3 kbp) for T. aestivum and 1.5 μm (4.7 kbp) for N. tabacum, respectively. This distribution is significantly different from that for small circular DNAS in mitochondria. Small polydisperse circular (spc) DNA/protein complexes were also observed by a rapid microscale method of mica-press-adsorption for electron microscopy. Complexes of spcDNA/protein showed a similar size distribution to cccDNA. The average number of spcDNA/protein complexes per nucleus was estimated at more than hundred. The origin and biological functions of the nuclear circular DNAs are discussed.
- 日本植物生理学会の論文
著者
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Kunisada Takahiro
Department of Tissue and Organ Development, Regeneration and Advanced Medical Science, Gifu Universi
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KINOSHITA Yasuhiro
Research Center, Nippon Paint Co., Ltd.
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YAMAGISHI Hideo
Department of Biophysics, Graduate School of Science, Kyoto University
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Yamagishi Hideo
Department Of Biophysics Faculty Of Science Kyoto University
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Yamada Yasuyuki
Research Centerfor Cell and Tissue Culture, Faculty of Agriculture, Kyoto University
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Ohnishi N
Kyoto Univ. Kyoto Jpn
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Kunisada T
Division Of Immunology Department Of Molecular And Cellular Biology School Of Life Science Faculty O
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Kunisada T
Kyoto Univ.
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Kunisada Takahiro
Department Of Immunology School Of Life Science Tottori University
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Kunisada Takahiro
Department Of Biophysics Faculty Of Science Kyoto University
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Ohnishi Noboru
Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University
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Ohnishi Noboru
Research Center For Cell And Tissue Culture Faculty Of Agriculture Kyoto University
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Kinoshita Yasuhiro
Research Center For Cell And Tissue Culture Faculty Of Agriculture Kyoto University
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Yamagishi H
Kyoto Univ.
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Yamada Yasuyuki
Research Center For Cell And Tissue Culture Faculty Of Agriculture Kyoto University
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YAMADA Yasuyuki
Research Center for Ceil and Tissue Culture, Faculty of Agriculture, Kyoto University
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