Uptake of Lysine by Wild-Type and S-(2-Aminoethyl)-L-cysteine-Resistant Suspension-Cultured Cells of Triticum aestivum

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The kinetics of uptake of L-lysine in wheat (Triticum aestivum cv. Chinese Spring) were analyzed in wild-type cells and in AEC-1 variant cells that are resistant to S-(2-aminoethyl)-L-cysteine (AEC). Uptake of lysine by AEC-1 cells was considerably slower than that by the wild-type cells. In the presence of carbonylcyanide m-chlorophenylhydrazone, the rates of uptake by both types of cell were reduced to a similar linear component. Fitting the uptake data to one linear (diffusional) component and one Michaelis-Menten (active) system showed that, as compared to wild-type cells, AEC-1 cells have a reduced V_<max> and an increased K_m with respect to the active . component, byt they have a similar diffusional component. Inhibition experiments with various amino acids indicated that the active component represents a carrier specific for basic amino acids, which was competitively inhibited by AEC. The AEC-1 cells also showed reduced uptake of several neutral and acidic amino acids, but the rate of uptake of 3-O-methylglucose was somewhat higher than that by wild-type cells.
日本植物生理学会の論文