Capillary Microinjection into Protoplasts and Intranuclear Localization of Injected Materials
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概要
- 論文の詳細を見る
By a direct microinjection through pressurized glass micropipettes, we injected fluorescent Lucifer Yellow, berberine and berberine-bound DNA into protoplasts isolated from cultured Euphorbia millii cells and from tobacco mesophyll cells. The protoplasts to be injected were held by a holding pipette with which the cytoplasm or nucleus of the protoplasts could be oriented with reference to the tip of the injection pipette. Upon microinjection of a berberine-bound DNA, the nucleus and intranuclear organelles, possibly including nucleolus, specifically exhibited yellow fluorescence due to berberine. This provides a direct evidence that DNA molecules can be microinjected into the intranuclear compartment of the protoplasts. The injected protoplasts survived the capillary microinjection.
- 日本植物生理学会の論文
著者
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Yamada Yasuyuki
Research Centerfor Cell and Tissue Culture, Faculty of Agriculture, Kyoto University
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Yamada Yasuyuki
Grad. School Of Biol. Sci. Naist
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Yamada Yasuyuki
Graduate School Of Biological Science Nara Institute Of Science And Technoiogy
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Morikawa Hiromichi
Research Center For Cell And Tissue Culture Faculty Of Agriculture Kyoto University
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Yamada Y
Present Address: Graduate School Of Biological Science Nara Institute Of Science And Technology
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Morikawa H
Hiroshima Univ. Higashi‐hiroshima Jpn
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Yamada Yasuyuki
Research Center For Cell And Tissue Culture Faculty Of Agriculture Kyoto University
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YAMADA Yasuyuki
Research Center for Ceil and Tissue Culture, Faculty of Agriculture, Kyoto University
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