Quantification of Physical and Cyto-physiological Conditions for the Electrofusion of Saccharomyces cerevisiae(Microbiology & Fermentation Industry)
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概要
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Various conditions for obtaining hybrids of the auxotrophic mutants SH1509 and SH1512 of Saccharomyces cerevisiae by electrofusion were investigated. An AC field of 400 V_p/cm and a DC field of 2 square pulses (7 kV/cm; 60 μsec each) at an interval of 0.5 sec were effective. Treatment with 0.2 (SH1509) or 1.0mg/ml (SH1512) Zymolyase for 1 or 1.5 hr was essential. As to the molarity of the osmotic stabilizer (sorbitol), the hybrid yield peaked at 0.6 M. The presence of CaCl_2 (up to 0.4 mM) or 0.1 mM CaCl_2 with 0.1 mM MgCl_2 enhanced the yield. The temperature of the spheroplast suspension during pulsations also affected the yield, the most suitable temperature being 28℃.
- 社団法人日本農芸化学会の論文
- 1990-08-23
著者
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Yamada Yasuyuki
Research Center For Cell And Tissue Culture Faculty Of Agriculture Kyoto University
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Togawa Yoshiyuki
Biotechnology Instruments Department Shimadzu Corporation
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NODA Kotaro
Biotechnology Instruments Department, Shimadzu Corporation
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Noda Kotaro
Biotechnology Instruments Department Shimadzu Corporation
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