Application of the Freeze-Blast Method to Disruption of Cultured Plant Cells

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概要

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• The freeze-blast method with a 'Cryo-Clean Blaster (CCB)' was applied to disrupt liquid-cultured plant cells of Lavandula vera L_<10> 4-2154. Recovery of peroxidase and chlorophylls a and b by this method was relatively higher than that obtained by the glass beads method. Under hypertonic conditions, chloroplasts were also isolated without lysis of the cells.

• 1990-02-25

著者

• Ueda Mitsuyoshi

  Department of Applied Biochemistry, Division of Applied Life Sciences, Graduate School of Agricultur

• Sato Fumihiko

  Research Centerfor Cell and Tissue Culture, Faculty of Agriculture, Kyoto University

• Yamada Yasuyuki

  Research Centerfor Cell and Tissue Culture, Faculty of Agriculture, Kyoto University

• Nakajima Hiroki

  Department Of Industrial Chemistry Faculty Of Engineering Kyoto University

• Yamada Yukio

  Fine Chemicals Research Laboratories Kaneka Corporation

• Tanaka Atsuo

  Department Of Applied Physics Faculty Of Engineering Osaka University

• UEDA Mitsuyoshi

  京都大学大学院工学研究科

• Yamada Yukio

  Mechanical Engineering Laboratory Material Science And Bioengineering Department Aist

• Sato Fumihiko

  Research Center For Cell And Tissue Culture Faculty Of Agriculture Kyoto University

• Nakajima H

  Basic Technology Department Research And Development Center Unitika Ltd.

• Tsumura Masanori

  Exploratory Research Laboratories:fujisawa Pharmaceutical Co. Ltd.

• OMORI YOSHIYUKI

  Tecnical Institute, Taiyo Sanso Co., Ltd.,

• TSUMURA MASANORI

  Tecnical Institute, Taiyo Sanso Co., Ltd.,

• ICHIDA TAIZO

  Tecnical Institute, Taiyo Sanso Co., Ltd.,

• Ichida Taizo

  Tecnical Institute Taiyo Sanso Co. Ltd.

• Omori Yoshiyuki

  Tecnical Institute Taiyo Sanso Co. Ltd.

• Yamada Yuko

  Kyoto Univ. Kyoto Jpn

• Yamada Yasuyuki

  Research Center For Cell And Tissue Culture Faculty Of Agriculture Kyoto University

• Nakajima Hiroki

  Department Of Diagnostic Pathology Gunma University Graduate School Of Medicine

• Sato Fumihiko

  Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University

• YAMADA Yasuyuki

  Research Center for Ceil and Tissue Culture, Faculty of Agriculture, Kyoto University

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