Construction of an Autonomously Replicating Plasmid in n-Alkane-Assimilating Yeast, Candida tropicalis
スポンサーリンク
概要
- 論文の詳細を見る
A transformation system using the autonomously replicating plasmid in the n-alkane-assimilating and asporogenic diploid yeast, Candida tropicalis, was developed. For the cloning of a DNA fragment containing a potential autonomously replicating sequence(ARS)from the genomic DNA of C.tropicalis, the ura3 mutant obtained using ethylmethane sulfonate as the host and the URA3 gene amplified by PCR using the C.tropicalis genomic DNA as a selectable marker were prepared. Comparison of ARSs among yeasts revealed that the consensus sequence found in S.cerevisiae was also present in C.tropicalis. The autonomously replicating plasmid containing the putative ARS as the shuttle vector, capable of replicating in both E.coli and C.tropicalis, was first constructed. The transformation system using this plasmid, in addition to the integrative transformation system, will be applicable to genetic studies of C.tropicalis.
- 社団法人日本生物工学会の論文
- 1999-06-25
著者
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Kamasawa N
日本女子大学理学部
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Matsui T
Pharmaceuticals & Biotechnology Laboratory Japan Energy Co.
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HARA AKIHIRO
Pharmaceuticals & Biotechnology Laboratory, Japan Energy Co.
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UEDA MITSUYOSHI
Laboratory of Applied Biological Chemistry, Department of Synthetic Chemistry and Biological Chemist
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MATSUI TORU
Pharmaceuticals & Biotechnology Laboratory, Japan Energy Co.
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FURUHASHI KEIZO
Pharmaceuticals & Biotechnology Laboratory, Japan Energy Co.
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KANAYAMA NAOKI
Laboratory of Applied Biological Chemistry, Department of Synthetic Chemistry and Biological Chemist
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TANAKA ATSUO
Laboratory of Applied Biological Chemistry, Department of Synthetic Chemistry and Biological Chemist
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Tanaka A
Department Of Etiology And Pathophysiology National Cardiovascular Center Research Institute
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Furuhashi Keizo
Pharmaceuticals & Biotechnology Laboratory Japan Energy Co.
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Tanaka A
京都大学大学院工学研究科
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UEDA Mitsuyoshi
京都大学大学院工学研究科
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Matsui Toru
Tsukuba Branch Of Bio-refining Process Laboratory Advanced Technology And Research Institute Petrole
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Tanaka A
Mie Univ. Mie Jpn
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Tanaka A
Kao Corp. Wakayama Jpn
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Hara Akihiro
Pharmaceuticals & Biotechnology Laboratory Japan Energy Co.
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Takagi Atsuko
Department Of Etiology And Pathophysiology National Cardiovascular Center Research Institute
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Tanaka Atsuo
Laboratory Of Applied Biological Chemistry Department Of Synthetic Chemistry And Biological Chamistr
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Ueda Mitsuyoshi
Laboratory Of Applied Biological Chemistry Department Of Synthetic Chemistry And Biological Chemistr
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Ueda Mitsuyoshi
Laboratory Of Applied Biological Chemistry Department Of Synthetic Chemistry And Biological Chemistr
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Tanaka Atsuo
Laboratory Of Industrial Biochemistry Department Of Industrial Chemistry Faculty Of Engineering Kyot
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