<PAPER>Protection of Green Fluorescent Protein(GFP) from Photobleaching on the Cell Surface of Saccharomyces cerevisiae
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概要
- 論文の詳細を見る
Jellyfish Aequorea victoria green fluorescent protein (GFP) and its variants are widely used in many biological studies. A lot of reports related in GFP has been appeared in this decade as a useful probe. For example, to prove a protein localization, transcriptional level, molecular interactions, etc. On the other hand, there are not so many reports using GFP in applicational reports like bioprocess. In late years, enhanced GFP (EGFP) was able to be displayed as active form on the surface of yeast Saccharomyces cerevisiae. This "arming yeast" is expected to be used in practical study and industry. However, it had better to be improved that fluorescence of displayed EGFP is not stable because cell surface contacts medium whose pH is changed drastically depending on growtb phase. We aimed to inhibit the photobleaching of EGFP on the cell surface as long as possible and it was succeeded by using HEPES in the medium.
- 神戸市立工業高等専門学校の論文
- 2002-03-01
著者
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Kamasawa N
日本女子大学理学部
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Tanaka A
Department Of Etiology And Pathophysiology National Cardiovascular Center Research Institute
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SHIBASAKI SEIJI
Department of Pharmacy, School of Pharmacy, Hyogo University of Health Sciences
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SHIBASAKI Seiji
応用化学科
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Tanaka A
京都大学大学院工学研究科
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HIRAYAMA Megumi
日本女子大学理学部
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IKEDA Yuriko
日本女子大学理学部
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KAMASAWA Naomi
日本女子大学理学部
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OSUMI Masako
日本女子大学理学部
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TANAKA Atsuo
京都大学大学院工学研究科
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UEDA Mitsuyoshi
京都大学大学院工学研究科
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Osumi Masako
Department Of Chemical And Biological Sciences Faculty Of Science Japan Women's University
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Osumi Masako
Laboratory Of Electron Microscopy/open Research Center Japan Women's University
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大隈 正子
日本女子大学理学部物質生物科学科
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Tanaka A
Mie Univ. Mie Jpn
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Tanaka A
Kao Corp. Wakayama Jpn
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大隅 正子
Department Of Chemical And Biological Sciences Faculty Of Science Japan Women's University
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大隅 正子
日本女子大・理・物生
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Takagi Atsuko
Department Of Etiology And Pathophysiology National Cardiovascular Center Research Institute
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Ueda Mitsuyoshi
Laboratory Of Applied Biological Chemistry Department Of Synthetic Chemistry And Biological Chemistr
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Tanaka Atsuo
Laboratory Of Industrial Biochemistry Department Of Industrial Chemistry Faculty Of Engineering Kyot
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