Analysis of Monoglyceride Synthetic Reaction in a Solvent-Free Two-Phase System Catalyzed by a Monoacylglycerol Lipase from Pseudomonas sp.LP7315
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概要
- 論文の詳細を見る
Kinetics of monoglyceride synthesis catalyzed by a monoacylglycerol lipase (MGL) isolated from Pseudomonas sp.Lp7315 was studied at 65℃ in a solvent-free two-phase system, in which fatty acid droplets were dispersed in a glycerol phase containing a small amount of water. The initial rate of the synthetic reaction depended on several factors: the amounts of fatty acid and glycerol, and the concentration of MGL in the glycerol phase. To analyze the effects of these factors, a kinetic model was developed based on the assumption that the adsorption equilibrium of MGL molecules at the interface between the two phases is the crucial factor for the synthetic reaction. The model was found to yield good approximations of the initial synthetic rate under various reaction conditions. The analysis suggests that the adsorption behavior of MGL onto the interface had a large effect on the initial rate of the monoglyceride synthesis.
- 社団法人日本生物工学会の論文
- 2001-01-25
著者
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Sakiyama Takaharu
Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University
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Nakanishi Kazuhiro
Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University
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崎山 高明
Department Of Bioscience And Biotechnology Faculty Of Engineering Okayama University:(present Addres
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Kusubayashi Noriko
Department Of Biochemical Engineering And Science Kyushu Institute Of Technology
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Tanaka A
Kao Corp. Wakayama Jpn
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YOSHIMI TSUYOSHI
Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University
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TANAKA ATSUSHI
Daiwa Kasei K.K.
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OZAKI SHO
Daiwa Kasei K.K.
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Sakiyama Takaharu
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Nakanishi Kazuhiro
Department Of Bioscience And Biotechnology Faculty Of Engineering Okayama University
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Nakanishi K
Department Of Bioscience And Biotechnology Faculty Of Engineering Okayama University
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Nakanishi Kazuhiro
Department Of Anesthesia Nippon Medical School
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Nakanishi Kazuhiro
Deparment Of Food Science And Technology Faculty Of Agriculture Kyoto University
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