Purification and Characterization of a Monoacylglycerol Lipase from Pseudomonas sp.LP7315
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概要
- 論文の詳細を見る
A monoacylglycerol lipase (MGL) was purified from Pseudomonas sp. LP7315 by ammonium sulfate precipitation, anion-exchange chromatography, and preparative electrophoresis. The purified enzyme was homogeneous on SDS-PAGE with a molecular mass of 59 kDa. Its hydrolytic activity was confirmed to be specific for monoglycerides: the enzyme did not hydrolyze di- and triglycerides. MGL was found to be stable even after 1-h incubation at 65℃. The optimum pH for monopalmitin hydrolysis was approximately 8. The hydrolytic activity depended not only on temperature and pH but also on the type of monoglyceride used. MGL also catalyzed monoglyceride synthesis at 65℃ in a solvent-free two-phase system, in which fatty acid droplets were dispersed in the glycerol phase with a low water content. The synthetic reaction proceeded at a constant rate for approximately 24 h and approximately reached an equilibrium after 48 h of reaction. The initial rate and equilibrium yield of the synthetic reaction depended on the type of fatty acid used as the substrate.
- 社団法人日本生物工学会の論文
- 2001-01-25
著者
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Sakiyama Takaharu
Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University
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Nakanishi Kazuhiro
Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University
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崎山 高明
Department Of Bioscience And Biotechnology Faculty Of Engineering Okayama University:(present Addres
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Kusubayashi Noriko
Department Of Biochemical Engineering And Science Kyushu Institute Of Technology
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Tanaka A
Kao Corp. Wakayama Jpn
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YOSHIMI TSUYOSHI
Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University
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TANAKA ATSUSHI
Daiwa Kasei K.K.
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OZAKI SHO
Daiwa Kasei K.K.
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MIYAKE AKIRA
Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University
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UMEOKA MIDORI
Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University
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Umeoka Midori
Department Of Bioscience And Biotechnology Faculty Of Engineering Okayama University
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Sakiyama Takaharu
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Nakanishi Kazuhiro
Department Of Bioscience And Biotechnology Faculty Of Engineering Okayama University
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Nakanishi K
Department Of Bioscience And Biotechnology Faculty Of Engineering Okayama University
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Nakanishi Kazuhiro
Department Of Anesthesia Nippon Medical School
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Miyake Akira
Department Of Bioscience And Biotechnology Faculty Of Engineering Okayama University
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Nakanishi Kazuhiro
Deparment Of Food Science And Technology Faculty Of Agriculture Kyoto University
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