Mutational Analysis of Uncharged Polar Residues and Proline in the Distal One-Third (Thr^<130>-Pro^<142>) of the Highly Conserved Region of Mouse Slc10a2
スポンサーリンク
概要
- 論文の詳細を見る
Solute carrier family member 2 (SLC10A2) reabsorbs bile acids at the distal terminus of the ileum in an Na+-dependent manner. Alignment of deduced amino acid sequences of SLC10 family members and homologous genes in various species revealed a highly conserved region that corresponds to Gly104–Pro142 of SLC10A2. To elucidate the functional importance of this region, uncharged polar residues and Pro in the distal one-third of this region in mouse Slc10a2 (mSlc10a2) were submitted to mutational analysis, and taurocholic acid uptake and cell surface localization were evaluated. In addition to mutations that abolished almost all of the transport activity with and without cellular localization failure (P142V and T130A respectively), a mutation that perhaps affected affinity for taurocholic acid was identified (T134A). These results suggest that the highly conserved region contains residues involved in the substrate interaction, function, and cellular localization of mSlc10a2.
- 社団法人 日本農芸化学会の論文
- 2009-07-23
著者
-
崎山 高明
Department Of Bioscience And Biotechnology Faculty Of Engineering Okayama University:(present Addres
-
Saeki Tohru
Laboratory of Molecular Nutrition, Kyoto Prefectural University
-
Mizushima Satoko
Laboratory of Molecular Nutrition, Kyoto Prefectural University
-
Ueda Kazumitsu
Division of Applied Life Science, Graduate School of Agriculture, Kyoto University
-
IWAMI Kimikazu
Laboratory of Molecular Nutrition, Kyoto Prefectural University
-
KANAMOTO Ryuhei
Laboratory of Molecular Nutrition, Kyoto Prefectural University
-
Iwami K
Lab. Of Molecular Nutrition Kyoto Prefectural Univ.
-
Saeki Tohru
Laboratory Of Molecular Nutrition Department Of Biological Resource Chemistry Kyoto Prefectural Univ
-
Iwami Kimikazu
Laboratory Of Food And Nutritional Sciences Department Of Agricultural Chemistry Kyoto Prefectural U
-
Kanamoto R
Lab. Of Molecular Nutrition Kyoto Prefectural Univ.
-
Kanamoto Ryuhei
Laboratory Of Molecular Nutrition Department Of Biological Resource Chemistry Kyoto Prefectural Univ
-
Ueda Kazumitsu
Inst. For Integrated Cell-material Sciences (icems) And Lab. Of Cellular Biochemistry Div. Of Applie
-
Saeki Tohru
Laboratory Of Molecular Nutrition Kyoto Prefectural University
-
Mizushima Satoko
Laboratory Of Molecular Nutrition Kyoto Prefectural University
-
KANAMOTO Ryuhei
Laboratory of Food and Nutrition Department of Agricultural Chemistry, Kyoto Prefectural University
関連論文
- 2E11-4 ポリスチレン親和性ペプチドを用いるタンパク質吸着の配向制御(生物化学工学,一般講演)
- 食品製造プロセスにおける新規洗浄方法の原理とその特性 (特集 食品工場の洗浄・除菌技術)
- S219 マイクロバイオリアクターとマイクロ生体反応素子の展望と課題(コンビナトリアル・バイオエンジニアリングの進展,シンポジウム)
- 306 二糖-高分子複合アモルファスマトリクスの諸物性の解析(培養工学,一般講演)
- 308 酵素の固体表面に対する付着特性の解析と付着の制御(培養工学,一般講演)
- 309 機能性タンパク質の固体表面への結合制御に関する基礎的研究(培養工学,一般講演)
- 310 H_2O_電気分解処理における金属表面からのタンパク質汚れの除去(培養工学,一般講演)
- 549 Streptomyces sp.由来新規アシラーゼの多様な基質特異性(酵素・酵素工学・タンパク質工学,一般講演)
- 食品製造プロセスで発生する汚れの新規洗浄方法 (特集:食品製造装置の洗浄殺菌)
- 656 H_2O_2-電気分解処理によるステンレス表面に付着したタンパク質汚れの除去
- 655 キトサン/コンドロイチン硫酸複合ゲルのpH応答型膨潤特性とその応用
- 413 膜面液体培養法を用いた遺伝子組換えAspergillus oryzaeによる酵素生産
- 247 Streptomyces sp.由来カプサイシン加水分解酵素の精製
- 246 トランスグルタミナーゼによるβ-ラクトグロブリンの脂肪酸修飾
- 1137 糖類アモルファスによる乾燥タンパク質の構造保持作用
- 942 複合体形成によるEscherichia coli由来セリンアセチルトランスフェラーゼの低温失活及びプロテアーゼに対する安定性の増加
- 540 アミノ酸およびペプチド類のステンレス粒子への吸脱着挙動
- 927 アミノアシラーゼを用いたカプサイシン誘導体の合成
- 929 水可溶性溶媒中でのN-アシルアミノ酸の酵素合成
- 928 アセチルCoA再生系を組み込んだシステイン合成酵素を用いる非天然アミノ酸合成
- 膜面液体培養法を用いた海洋植物プランクトンOscillatoria属の培養と特性解明
- Adsorption Characteristics of Oligopeptides Composed of Acidic and Basic Amino Acids on Titanium Surface(BIOCHEMICAL ENGINEERING)
- Protease Susceptibility of β-Lactoglobulin Adsorbed on Stainless Steel Surface as Evidence of Contribution of Its Specific Segment to Adsorption(MISCELLANEOUS)
- 1019 膜面液体培養(MSLC)を用いたMonascus pilosus kによる色素生産
- 1421 タンパク質・ペプチド・アミノ酸のステンレス表面への付着特性の解析
- 食品製造プロセスにおける汚れの付着と洗浄
- Cleaning of a Stainless Steel Surface Fouled with Protein Using a UV-H_2O_2 Technique
- Characteristics of α-Glucosidase Production from Recombinant Aspergillus oryzae by Membrane-Surface Liquid Culture in Comparison with Various Cultivation Methods(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Production of Nonproteinaceous Amino Acids Using Recombinant Escherichia coli Cells Expressing Cysteine synthase and Related Enzymes with or without the Secretion of O-Acetyl-L-Serine (MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- サーモライシンの有機溶媒への懸濁方法が酵素活性に及ぼす影響
- 850 アセチルCoA再生系を組込んだシステインシンテターゼを用いたβ-ピラゾールアラニンの合成
- 822 大腸菌由来システイン合成酵素の特性と速度論的解析
- 817 C-末端欠失セリンアセチルトランスフェラーゼの精製と諸性質
- 429 Escherichia coli由来セリンアセチルトランスフェラーゼの精製と速度論的解析
- Evidence for the Existence of a Soybean Resistant Protein That Captures Bile Acid and Stimulates Its Fecal Excretion
- Mutational Analysis of Uncharged Polar Residues and Proline in the Distal One-Third (Thr130–Pro142) of the Highly Conserved Region of Mouse Slc10a2
- Effects of Ala Substitution for Conserved Cys Residues in Mouse Ileal and Hepatic Na+-Dependent Bile Acid Transporters
- Adsorption Characteristics of Tryptic Fragments of Bovine β-Lactoglobulin on a Stainless Steel Surface
- Fouling and Cleaning of Stainless Steel Surface : Adsorption and Desorption Behavior of Bovine Serum Albumin and Gelatin
- Performance of Protease as a Cleaning Agent for Stainless Steel Surfaces Fouled with Protein
- Vinexin, CAP/ponsin, ArgBP2 : a Novel Adaptor Protein Family Regulating Cytoskeletal Organization and Signal Transduction
- 1020 膜面液体培養(MSLC)を用いた遺伝子組換えAspergillus oryzaeによる酵素生産と特性解明
- Increase in the Stability of Serine Acetyltransferase from Escherichia coli against Cold Inactivation and Proteolysis by Forming a Bienzyme Complex
- On the Adsorption of Proteins on Solid Surfaces, a Common but Very Complicated Phenomenon
- Analysis of Monoglyceride Synthetic Reaction in a Solvent-Free Two-Phase System Catalyzed by a Monoacylglycerol Lipase from Pseudomonas sp.LP7315
- Purification and Characterization of a Monoacylglycerol Lipase from Pseudomonas sp.LP7315
- Characteristics of Serine Acetyltransferase from Escherichia coli Deleting Different Lengths of Amino Acid Residues from the C-Terminus
- Effects of Bienzyme Complex Formation of Cysteine Synthetase from Escherichia coli on Some Properties and Kinetics
- Kinetic Analysis of Synthesis of a Dipeptide Precursor Using an Immobilized Enzyme in Water-Immiscible Organic Solvents
- Kinetics and Equilibrium for Thermolysin-Catalyzed Syntheses of Dipeptide Precursors in Aqueous/Organic Biphasic Systems
- Development of Novel Advanced Oxidation System Using Combined UV/H_2O_2 Technique and Kinetic Analysis for Decomposition of Dye Solutions
- バイオ・食品製造プロセスにおける汚れの付着と洗浄 (特集 最近の生物工学の展望)
- Analysis for Partition Equilibrium of Amino Acid Derivatives in Aqueous/Organic Biphasic Systems
- Expression of Human P-Glycoprotein in Yeast Cells : Effects of Membrane Component Sterols on the Activity of P-Glycoprotein(Biological Chemistry)
- Production of a Site Specifically Cleavable P-Glycoprotein-β-galactosidase Fusion Protein(Biological Chemistry)
- Postprandial Changes in Ornithine Decarboxylase Activity, and the Mucosal and Intraluminal Polyamine Levels in the SmalI Intestine of Rats Concerning the Significance of Intestinal Putrescine Absorption
- Decreased Antioxidative Activity of Maize Zein in Response to Deamidation Rate
- Gastrointestinal Transit of Soybean Trypsin Inhibitor and Fluctuations in Intraluminal Trypsin
- Development of Cylindrical Apparatus for Membrane-Surface Liquid Culture and Production of Kojic Acid Using Aspergillus oryzae NRRL484
- Development of a Model for Analyzing the Swelling Rate of Ionic Gels on the Basis of the Diffusion of Mobile Ions: Application to the pH-Sensitive Swelling of a Polyelectrolyte Complex Gel Prepared from Xanthan and Chitosan
- Analysis of the pH-Sensitive Swelling Rate of a Polyelectrolyte Complex Gel Prepared from Xanthan and Chitosan by the Collective Diffusion Model
- Soybean Resistant Proteins Interrupt an Enterohepatic Circulation of Bile Acids and Suppress Liver Tumorigenesis Induced by Azoxymethane and Dietary Deoxycholate in Rats
- Non-involvement of the K-ras Mutation in Colon Carcinogenesis Promoted by Dietary Deoxycholate in Azoxymethane-treated Rats
- Cholesterol and Plant Sterol Efflux from Cultured Intestinal Epithelial Cells Is Mediated by ATP-Binding Cassette Transporters
- Human MDR1 and MRP1 Recognize Berberine as Their Transport Substrate
- Molecular Mechanisms of Subcellular Localization of ABCGS and ABCG8
- Mutational Analysis of Uncharged Polar Residues and Proline in the Distal One-Third (Thr^-Pro^) of the Highly Conserved Region of Mouse Slc10a2
- Soybean Resistant Protein Elevates Fecal Excretion of Cholesterol and Bile Acids and Decreases Hepatic Cholesterol Content in Comparison with Soybean Protein Isolate
- Effects of Ala Substitution for Conserved Cys Residues in Mouse Ileal and Hepatic Na^+-Dependent Bile Acid Transporters
- Taurocholic Acid Does Not Induce Apoptosis in HCT116 Cells Regardless of Its Intracellular Concentration
- Characteristics of Apoptosis in HCT116 Colon Cancer Cells Induced by Deoxycholic Acid
- Inverse Correlation between the Nitrogen Balance and Induction of Rat Liver Serine Dehydratase (SDH) by Dietary Protein
- Postprandial Changes in Portal Venous Free Amino Acids and Insulin/Glucagon Ratios as the Result of Protein Over-Intake Are Not Directly Linked to Serine Dehydratase Induction in Rat Liver Irrespective of Age
- Response of the Induction of Rat Liver Serine Dehydratase to Changes in the Dietary Protein Requirement(Food & Nutrition Science)
- Quantity as Well as Quality of Dietary Protein Affects Serine Dehydratase Gene Expression in Rat Liver
- Characterization of Cloned Mouse Na^+/taurocholate Cotransporting Polypeptide by Transient Expression in COS-7 Cells(Biochemistry & Molecular Biology)
- Effects of Cys Mutation on Taurocholic Acid Transport by Mouse Ileal and Hepatic Sodium-dependent Bile Acid Transporters(Biochemistry & Molecular Biology)
- Antitumorigenic Effects of Several Food Proteins in a Rat Model with Colon Cancer and Their Reverse Correlation with Plasma Bile Acid Concentration
- Preventive Effect of Soybean Resistant Proteins against Experimental Tumorigeneses in Rat Colon
- Postprandial Changes in the Function of Digestive Organs in Rats Meal-fed Twice a Day with 40% Casein-based and Protein-free Diets
- Some Rats Prefer a Non-protein Diet to a Protein-rich Diet when Given Free Access to Them
- Two-dimensional Analysis of Voluntary Locomotor Activity in Rats; Compact Apparatus and Its Application from Nutritional Aspects
- Influence of Intermittent Protein Intake Every Few Days on Rat Growth and Metabolic Function
- Segregated Intake of Protein-rich and -free Diets in Two Meals a Day; Its Effect on Growth and Vital Functions in Rats(Food & Nutrition)
- Nutritive Effect of Alternate Daily Ingestion of High- and Non-protein Diets in Growing Rats(Food & Nutrition)
- Cholesterol and Plant Sterol Efflux from Cultured Intestinal Epithelial Cells Is Mediated by ATP-Binding Cassette Transporters
- Soybean Curd Refuse Alleviates Experimental Tumorigenesis in Rat Colon
- Characterization, cDNA Cloning, and Fuctional Expression of Mouse Ileal Sodium-Dependent Bile Acid Transporter
- Feeding Soybean Resistant Protein to Rats Raises Fecal Bile Acid Excretion but Counteracts a Deoxycholate-Caused Decrease in Colonic Aberrant Crypt Foci
- Antioxidative Activity of Barley Hordein and Its Loss by Deamidation
- Purification and Characterization of Serine Acetyltransferase from Escherichia coli Partially Truncated at the C-Terminal Region
- デンプンを原料とする糸状菌の膜面液体培養法による機能性食品素材の生産
- 1018 膜面液体培養法(MSLC)を用いたAspergillue oryzae NRRL 484によるコウジ酸生産と特性解明
- 324 膜面液体培養法によるカビの培養とそのスケールアップ
- Stability of Immobilized Thermolysin in Organic Solvents
- Protein-Nutritive Assessment of Sake Lees Obtained by Brewing from Liquefied Rice
- Quantity as Well as Quality of Dietary Protein Affects Serine Dehydratase Gene Expression in Rat Liver
- 食品製造・バイオプロセスにおける洗浄とタンパク性汚れの付着(殺菌・洗浄のバイオテクノロジー)
- 新規に開発したカビの膜面液体培養法を用いた食品加工用酵素の生産に関する基礎研究
- 食品製造プロセスにおける汚れの付着とその洗浄