Protease Susceptibility of β-Lactoglobulin Adsorbed on Stainless Steel Surface as Evidence of Contribution of Its Specific Segment to Adsorption(MISCELLANEOUS)

スポンサーリンク

概要

• 論文の詳細を見る

• β-Lactoglobulin (β-Lg) is a major constituent of fouling deposits in the dairy industry. To determine the interaction between β-Lg and stainless steel surfaces, β-Lg irreversibly adsorbed on stainless steel particles was subjected to lysyl endopeptidase treatment and the course of fragmentation was compared with that observed for β-Lg in solution. The results showed a distinct difference between the courses of fragmentation: a fragment (residues 102-135) was liberated readily from β-Lg in solution but scarcely from β-Lg irreversibly adsorbed on stainless steel particles. This result strongly suggests that residues 102-135 include a segment primarily responsible for the interaction of β-Lg with stainless steel surfaces. This supports our previous results [Sakiyama et al., J. Biosci. Bioeng., 88, 536-541 (1999)] that showed that residues 125-135 of β-Lg have a strong affinity toward stainless steel surfaces and probably a major contribution to the adsorption of β-Lg.

• 社団法人日本生物工学会の論文
• 2006-05-25

著者

• 今村 維克

  岡山大学大学院自然科学研究科機能分子化学専攻

• Nakanishi Kazuhiro

  Graduate School of Natural Science and Technology, Okayama University

• Imamura Koreyoshi

  Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University

• Sakiyama Takaharu

  Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University

• Nakanishi Kazuhiro

  Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University

• Aya Atsuko

  Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University

• Embutsu Mari

  Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University

• 崎山 高明

  Department Of Bioscience And Biotechnology Faculty Of Engineering Okayama University:(present Addres

• Kusubayashi Noriko

  Department Of Biochemical Engineering And Science Kyushu Institute Of Technology

• Aya Atsuko

  Department Of Bioscience And Biotechnology Faculty Of Engineering Okayama University

• Embutsu Mari

  Department Of Bioscience And Biotechnology Faculty Of Engineering Okayama University

• Sakiyama Takaharu

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• Nakanishi Kazuhiro

  Department Of Bioscience And Biotechnology Faculty Of Engineering Okayama University

• Nakanishi K

  Department Of Bioscience And Biotechnology Faculty Of Engineering Okayama University

• Nakanishi Kazuhiro

  Division Of Chemistry And Biochemistry Graduate School Of Natural Science And Technology Okayama Uni

• Nakanishi Kazuhiro

  Department Of Anesthesia Nippon Medical School

• Saeki Tohru

  Laboratory Of Molecular Nutrition Kyoto Prefectural University

• Imamura Koreyoshi

  Graduate School Of Natural Science And Technology Okayama University

• Imamura Koreyoshi

  Department Of Bioscience And Biotechnology Faculty Of Engineering Okayama University

• Nakanishi Kazuhiro

  Graduate School Of Natural Science And Technology Okayama University

• Nakanishi Kazuhiro

  Deparment Of Food Science And Technology Faculty Of Agriculture Kyoto University

関連論文

• Cultivation characteristics and gene expression profiles of Aspergillus oryzae by membrane-surface liquid culture, shaking-flask culture, and agar-plate culture(BIOCHEMICAL ENGINEERING)
• Development of a highly efficient indigo dyeing method using indican with an immobilized β-glucosidase from Aspergillus niger(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• 3Ga07 ヒトFOXP3-DNA相互作用の迅速検出システムの構築(酵素学,酵素工学,タンパク質工学,糖鎖工学,一般講演)
• 2Ip05 クッションタンパク質を利用したペプチドータンパク質間相互作用検出系の検討(バイオセンシング・分析化学,一般講演)
• 2Ea05 オートトランスポーターを用いたペプチド細胞表層提示系の検討(タンパク質工学,一般講演)
• 2Da01 放線菌Streptomyces mobaraensis由来新規アミノアシラーゼの発現系の構築および特性解析(酵素学・酵素工学,一般講演)
• 酵素糖化と発酵を併用した小麦フスマからの効率的エタノール生産
• 2Aa03 組換えモノグリセリドリパーゼの精製及び生化学的特性解析(プロセス工学,一般講演)
• 3Da04 クッションタンパク質を用いたバイオ分子固定化技術(分析化学・物理化学,一般講演)
• 新規アシラーゼを用いた食品タンパク質のプロテアーゼ消化ペプチド断片への脂肪酸付加と機能性の調査
• 1C09-2 オートトランスポーターを用いた細胞表層提示系の構築におけるシグナルペプチドの影響(酵素学・酵素工学・タンパク質工学,一般講演)
• 1C11-1 放線菌Streptomyces mobaraensis由来長鎖および短鎖アミノアシラーゼの特性解析およびクローニング(酵素学・酵素工学・タンパク質工学,一般講演)
• 1C11-2 放線菌Streptomyces mobaraensis由来ペニシリン V アシラーゼのアシル基転移反応を利用した機能性化合物の酵素合成(酵素学・酵素工学・タンパク質工学,一般講演)
• 2F14-4 膜面液体培養法と振盪フラスコ培養法を用いた麹菌のプロテアーゼ生産及び遺伝子発現解析(培養工学,センサー・計測工学・ロボット工学,プロセス工学,一般講演)
• 2E11-4 ポリスチレン親和性ペプチドを用いるタンパク質吸着の配向制御(生物化学工学,一般講演)
• 食品製造プロセスにおける新規洗浄方法の原理とその特性 (特集 食品工場の洗浄・除菌技術)
• 酵素法による脂質付加食品素材物質の大量合成法の開発と機能性の解明
• S219 マイクロバイオリアクターとマイクロ生体反応素子の展望と課題(コンビナトリアル・バイオエンジニアリングの進展,シンポジウム)
• 306 二糖-高分子複合アモルファスマトリクスの諸物性の解析(培養工学,一般講演)
• 308 酵素の固体表面に対する付着特性の解析と付着の制御(培養工学,一般講演)
• 309 機能性タンパク質の固体表面への結合制御に関する基礎的研究(培養工学,一般講演)
• 310 H_2O_電気分解処理における金属表面からのタンパク質汚れの除去(培養工学,一般講演)
• 549 Streptomyces sp.由来新規アシラーゼの多様な基質特異性(酵素・酵素工学・タンパク質工学,一般講演)
• 2.最近の洗浄・殺菌技術の動向 : 2.3 過酸化水素の電気分解を利用した新規洗浄方法
• 食品製造プロセスで発生する汚れの新規洗浄方法 (特集:食品製造装置の洗浄殺菌)
• 656 H_2O_2-電気分解処理によるステンレス表面に付着したタンパク質汚れの除去
• 655 キトサン/コンドロイチン硫酸複合ゲルのpH応答型膨潤特性とその応用
• 413 膜面液体培養法を用いた遺伝子組換えAspergillus oryzaeによる酵素生産
• MN-P23 Development of peptide immobilization method using PS-tagged cushion protein(Section X Micro/Nano Technology for Analysis and Cell Manipulation)
• 2007年度日本食品工学会奨励賞 過酸化水素の電気分解を利用した金属表面の洗浄技術であるH2O2-電気分解洗浄の洗浄特性に及ぼす諸因子の影響
• Adsorption Characteristics of Oligopeptides Composed of Acidic and Basic Amino Acids on Titanium Surface(BIOCHEMICAL ENGINEERING)
• Identification of Genes from Aspergillus oryzae That Are Preferentially Expressed in Membrane-Surface Liquid Culture(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Protease Susceptibility of β-Lactoglobulin Adsorbed on Stainless Steel Surface as Evidence of Contribution of Its Specific Segment to Adsorption(MISCELLANEOUS)
• Purification and Characterization of a Novel Aminoacylase from Streptomyces mobaraensis
• 3P-1085 機能的ペプチド固定化法を利用したCysteine Synthase複合体形成における機能性残基の同定(2bタンパク質工学,一般講演,酵素学,タンパク質工学および酵素工学,伝統の技と先端科学技術の融合)
• Cleaning of a Stainless Steel Surface Fouled with Protein Using a UV-H_2O_2 Technique
• Characteristics of α-Glucosidase Production from Recombinant Aspergillus oryzae by Membrane-Surface Liquid Culture in Comparison with Various Cultivation Methods(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Production of Nonproteinaceous Amino Acids Using Recombinant Escherichia coli Cells Expressing Cysteine synthase and Related Enzymes with or without the Secretion of O-Acetyl-L-Serine (MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Water Sorption and Glass Transition of Amorphous Sugars Containing BSA
• Relation between Thermal Stabilizing Effect of Sucrose on LDH and Sucrose-LDH Hydrogen Bond
• Influence of Protein on Phase Transition of Amorphous Sugar
• 2P-1103 オートトランスポーターを用いた大腸菌細胞表層提示系におけるアミノ酸配列の影響(2bタンパク質工学,一般講演,酵素学,タンパク質工学および酵素工学,伝統の技と先端科学技術の融合)
• 糖類アモルファスマトリクスにおける糖-タンパク質間相互作用とタンパク質の安定化(セミナー「食品,医薬品におけるアモルファス(非晶質)科学の応用と発展」)
• Effect of Oligosaccharides on Glucose Consumption by Rhodobacter sphaeroides in Polyhydroxyalkanoate Production from Enzymatically Treated Crude Sago Starch
• Effect of Organic Acid Profiles during Anaerobic Treatment of Palm Oil Mill Effluent on the Production of Polyhydroxyalkanoates by Rhodobacter sphaeroides
• Acetic Acid Separation from Anaerobically Treated Palm Oil Mill Effluent by Ion Exchange Resins for the Production of Polyhydroxyaikanoate by Alcaligenes eutrophus
• 食品の物性そして水(11)糖類アモルファスマトリクスに収着した水の状態および機能の非単一性
• Adsorption Characteristics of Tryptic Fragments of Bovine β-Lactoglobulin on a Stainless Steel Surface
• Fouling and Cleaning of Stainless Steel Surface : Adsorption and Desorption Behavior of Bovine Serum Albumin and Gelatin
• Performance of Protease as a Cleaning Agent for Stainless Steel Surfaces Fouled with Protein
• Comparative Study on Caustic and Enzymatic Cleanings of Stainless Steel Surface Fouled with β-Lactoglobulin
• Adsorption of β-Lactoglobulin onto the Surface of Stainless Steel Particles
• The Production of Polyhydroxyalkanoate from Anaerobically Treated Palm Oil Mill Effluent by Rhodobacter sphaeroides
• Increase in the Stability of Serine Acetyltransferase from Escherichia coli against Cold Inactivation and Proteolysis by Forming a Bienzyme Complex
• On the Adsorption of Proteins on Solid Surfaces, a Common but Very Complicated Phenomenon
• Analysis of Monoglyceride Synthetic Reaction in a Solvent-Free Two-Phase System Catalyzed by a Monoacylglycerol Lipase from Pseudomonas sp.LP7315
• Purification and Characterization of a Monoacylglycerol Lipase from Pseudomonas sp.LP7315
• Characteristics of Serine Acetyltransferase from Escherichia coli Deleting Different Lengths of Amino Acid Residues from the C-Terminus
• Effects of Bienzyme Complex Formation of Cysteine Synthetase from Escherichia coli on Some Properties and Kinetics
• Kinetic Analysis of Synthesis of a Dipeptide Precursor Using an Immobilized Enzyme in Water-Immiscible Organic Solvents
• Kinetics and Equilibrium for Thermolysin-Catalyzed Syntheses of Dipeptide Precursors in Aqueous/Organic Biphasic Systems
• Development of Novel Advanced Oxidation System Using Combined UV/H_2O_2 Technique and Kinetic Analysis for Decomposition of Dye Solutions
• Analysis for Partition Equilibrium of Amino Acid Derivatives in Aqueous/Organic Biphasic Systems
• Production of Kojic Acid by Membrane-Surface Liquid Culture of Aspergillus oryzae NRRL484
• Production of Neutral Protease by Membrane-Surface Liquid Culture of Aspergillus oryzae IAM2704
• Chromatographic Separation of Galactosylkojic Acid
• 3P-1055 Multiformity of β-Galactosidase Produced from Bacillus circulans
• Enzymatic Synthesis of Galactosylkojic Acid with Immobilized β-Galactosidase from Bacillus circulans
• Some Factors Affecting the Formation of γ-Cyclodextrin Using Cyclodextrin Glycosyltransferase from Bacillus sp. AL-6
• Purification and Properties of Cyclodextrin Glycosyltransferase from Bacillus sp. AL-6
• Systemic ATP infusion improves spontaneous pain and tactile allodynia, but not tactile hypesthesia, in patients with postherpetic neuralgia
• Lipase-Catalyzed Synthesis of Monolauroyl Maltose through Condensation of Maltose and Lauric Acid
• Condensation of L-Ascorbic Acid and Medium-Chain Fatty Acids by Immobilized Lipase in Acetonitrile with Low Water Content
• Inhibitory Activity of Alginates against the Formation of Calcium Phosphate
• Development of Cylindrical Apparatus for Membrane-Surface Liquid Culture and Production of Kojic Acid Using Aspergillus oryzae NRRL484
• Effect of Nasal Continuous Positive Airway Pressure on Pulmonary Edema Complicating Acute Myocardial Infarction
• Development of a Model for Analyzing the Swelling Rate of Ionic Gels on the Basis of the Diffusion of Mobile Ions: Application to the pH-Sensitive Swelling of a Polyelectrolyte Complex Gel Prepared from Xanthan and Chitosan
• Analysis of the pH-Sensitive Swelling Rate of a Polyelectrolyte Complex Gel Prepared from Xanthan and Chitosan by the Collective Diffusion Model
• Method for Analyzing pH-Sensitive Swelling of Amphoteric Hydrogels : Application to a Polyelectrolyte Complex Gel Prepared from Xanthan and Chitosan
• Cloning and Expression of a β-Galactosidase Gene of Bacillus circulans
• pH-Sensitive Swelling of a Polyelectrolyte Complex Gel Prepared from Xanthan and Chitosan
• Effects of Air and Water Contents on the Effective Thermal Conductivity of Air-impregnated Gels(Food & Nutrition)
• Intrinsic Thermal Conductivity of Gelatin Estimated Independently of Heat Conduction Models(Food & Nutrition)
• Synthesis of Peptides Consisting of Essential Amino acids by a Reactor System Using Three Proteinases and an Organic Solvent(Microbiology & Fermentation Industry)
• TEMPERATURE DEPENDENCE OF THE EFFECTIVE THERMAL CONDUCTIVITY OF FOOD GELS IMPREGNATED WITH AIR BUBBLES
• Enzymatic Synthesis of β-Lactam Antibiotics and N-Fatty-Acylated Amino Compounds by the Acyl-Transfer Reaction Catalyzed by Penicillin V Acylase from Streptomyces mobaraensis
• Filtration Characteristics and Structure of Cake in Crossflow Filtration of Bacterial Suspension
• Causes of the Production of Multiple Forms of β-Galactosidase by Bacillus circulans
• Purification, Characterization, Molecular Cloning, and Expression of a New Aminoacylase from Streptomyces mobaraensis : That Can Hydrolyze N-(Middle/Long)-chain-fatty-acyl-L-amino Acids as Well as N-Short-chain-acyl-L-amino acids
• Adsorption Characteristics of Various Proteins to a Titanium Surface(BIOCHEMICAL ENGINEERING)
• Stability of Immobilized Thermolysin in Organic Solvents
• Cloning and Expression of a β-Galactosidase Gene of Bacillus circulans
• 2Ep03 機能的タンパク質固定化技術を利用したNFkB(p50)阻害性ペプチドの探索(タンパク質工学/核酸工学/ペプチド工学,一般講演)
• 糖類アモルファスマトリクスの温度走査フーリエ変換赤外分光分析 : 相互作用状態の温度依存性
• 11p04 大腸菌を宿主としたStreptomyces mobaraensis由来ε-lysine acylase大量発現系の検討(生物化学工学,一般講演)
• 糖類アモルファスマトリクスに収着した水の状態および機能の非単一性
• 4Cp03 転写因子FOXP3を標的とした部位特異的ペプチド薬剤スクリーニング技術の検討(タンパク質工学/核酸工学,一般講演)
• 4Cp02 機能的バイオ分子固定化技術を利用したNFkB(p50)親和性ペプチドの探索(タンパク質工学/核酸工学,一般講演)
• 2007年度日本食品工学会奨励賞 過酸化水素の電気分解を利用した金属表面の洗浄技術であるH2O2-電気分解洗浄の洗浄特性に及ぼす諸因子の影響
• The Discoidin Domain of Bacillus circulans β-Galactosidase Plays an Essential Role in Repressing Galactooligosaccharide Production

もっと見る 閉じる

スポンサーリンク