Purification and Properties of Cyclodextrin Glycosyltransferase from Bacillus sp. AL-6
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概要
- 論文の詳細を見る
Cyclodextrin glycosyltrans ferase (EC2.4.1.19) from Bacillus sp. AL-6 was purified to a homogeneous protein, using ammonium sulfate fractionation, starch adsorption and ion-exchange chromatography on DEAE-Sephadex A-50. The enzyme, a monomeric protein with a molecular weight of 74,000,was determined by gel chromatography on Sephadex G-150 and SDS polyacrylamide gel electrophoresis. With soluble starch or potato starch as a substrate, the enzyme produced a considerable amount of γ-cyclodextrin with β-cyclodextrin. At the initial stage of the reaction, γ-cyclodextrin was predominant, and the amount of β-cyclodextrin increased gradually with time from the start of the reaction. The optimum pH for the formation of γ-cyclodextrin was 7 to 10 and the optimum temperature was 60℃. The addition of ethanol to the reaction mixture enhanced the formation of γ-cyclodextrin. When 4.4% soluble starch was used as the substrate with 20% ethanol, the maximum yield of γ-cyclodextrin was about 22% in comparison to 8% in the absence of ethanol. With 1.8% potato starch, the maximum yield was 25% at an ethanol concentration of 20%.
- 社団法人日本生物工学会の論文
- 1990-09-25
著者
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TOMITA Kenji
Department of Psychiatry, Imaise Branch, Ichinomiya City Hospital
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Nakanishi Kazuhiro
Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University
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Tomita K
Kyowa Hakko Kogyo Co. Ltd. Yamaguchi Jpn
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Kusubayashi Noriko
Department Of Biochemical Engineering And Science Kyushu Institute Of Technology
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Tomita Kenji
Department Of Biotechnology Faculty Of Engineering Okayama University
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Nakanishi K
Department Of Bioscience And Biotechnology Faculty Of Engineering Okayama University
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Nakanishi Kazuhiro
Department Of Anesthesia Nippon Medical School
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FUJITA YOSHITO
Fukui Food Processing Research Institute
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TSUBOUCHI HITOSHI
Fukui Food Processing Research Institute
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INAGI YUKIO
Fukui Food Processing Research Institute
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OZAKI AKIRA
Daiwa Kasei K.K.
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Tsubouchi Hitoshi
Department Of Bioscience Faculty Ofbiotechnology Fukui Prefectural University
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Tomita Kenji
Department Of Biotechnology And Life Science Tokyo University Of Agriculture And Technology
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Nakanishi Kazuhiro
Deparment Of Food Science And Technology Faculty Of Agriculture Kyoto University
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