Purification and Properties of a Cyclodextrin Glucanotransferase from Bacillus autolyticus 11149 and Selective Formation of β-Cyclodextrin
スポンサーリンク
概要
- 論文の詳細を見る
A cyclodextrin glucanotransferase (EC 2.4.1.19) (CGTase) from Bacillus autolyticus was purified to homogeneous, and its properties were investigated. The molecular weight was estimated to be 68,000 by gel chromatography and 70,000 by SDS-PAGE. optimum pH of the enzyme was 5.0-6.0. The optimum reaction temperature was 60℃. HgCl_2 inhibited the enzyme activity completely. The enzyme formed mainly β-cyclodextrin (β-CD) with a small amount of α-cyclodextrin (α-CD) and γ-cyclodextrin (γ-CD) from potato starch. Ethanol and Triton X-100 enhanced the specificity and yield of β-CD production. Especially, the effect of Triton X-100 was remarkable. With 4% (v/v) Triton X-100,β-CD was formed in a maximum yield of 56% from 10% (w/v) potato starch with negligible amounts of α-CD and γ-CD (both yields were less than 1%) at a later stage of the reaction.
- 1993-02-25
著者
-
Tomita Kenji
Department Of Biotechnology Faculty Of Engineering Okayama University
-
Tomita Kenji
Department Of Biotechnology And Life Science Tokyo University Of Agriculture And Technology
-
Nakanishi Kazuhiro
Deparment Of Food Science And Technology Faculty Of Agriculture Kyoto University
-
KANEDA MITSUTOSHI
Department of Biotechnology, Faculty of Engineering, Okayama University
-
KAWAMURA KICHIYA
Nakano Vinegar Co., Ltd.,
-
Kawamura Kichiya
Nakano Vinegar Co. Ltd.
-
Kaneda Mitsutoshi
Department Of Biotechnology Faculty Of Engineering Okayama University
関連論文
- 老人性認知症治療病棟における身体合併症について : 12ヶ月間の調査
- Adsorption Characteristics of Oligopeptides Composed of Acidic and Basic Amino Acids on Titanium Surface(BIOCHEMICAL ENGINEERING)
- Identification of Genes from Aspergillus oryzae That Are Preferentially Expressed in Membrane-Surface Liquid Culture(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Protease Susceptibility of β-Lactoglobulin Adsorbed on Stainless Steel Surface as Evidence of Contribution of Its Specific Segment to Adsorption(MISCELLANEOUS)
- Purification and Characterization of a Novel Aminoacylase from Streptomyces mobaraensis
- Cleaning of a Stainless Steel Surface Fouled with Protein Using a UV-H_2O_2 Technique
- Characteristics of α-Glucosidase Production from Recombinant Aspergillus oryzae by Membrane-Surface Liquid Culture in Comparison with Various Cultivation Methods(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Production of Nonproteinaceous Amino Acids Using Recombinant Escherichia coli Cells Expressing Cysteine synthase and Related Enzymes with or without the Secretion of O-Acetyl-L-Serine (MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Purification and Properties of γ-Glutamyltranspeptidase from Penicillium roqueforti IFO 4622
- Purification and Properties of Glutaminase from Aspergillus oryzae
- Synthesis of γ-Glutamyltaurine by γ-Glutamyltranspeptidase of Penicillium roqueforti(Microbiology & Fermentation Industry)
- Formation of γ-Glutamyl Peptides by Glutaminase of Aspergillus oryzae(Microbiology & Fermentation Industry)
- Action of Glutaminase in a Model System of a Soy Sauce Fermentation(Microbiology & Fermentation Industry)
- Distribution and Properties of γ-Glutamyltranspeptidase in Filamentous Fungi (Microbiology & Fermentation Industry)
- Effect of Oligosaccharides on Glucose Consumption by Rhodobacter sphaeroides in Polyhydroxyalkanoate Production from Enzymatically Treated Crude Sago Starch
- Effect of Organic Acid Profiles during Anaerobic Treatment of Palm Oil Mill Effluent on the Production of Polyhydroxyalkanoates by Rhodobacter sphaeroides
- Acetic Acid Separation from Anaerobically Treated Palm Oil Mill Effluent by Ion Exchange Resins for the Production of Polyhydroxyaikanoate by Alcaligenes eutrophus
- Adsorption Characteristics of Tryptic Fragments of Bovine β-Lactoglobulin on a Stainless Steel Surface
- Fouling and Cleaning of Stainless Steel Surface : Adsorption and Desorption Behavior of Bovine Serum Albumin and Gelatin
- Performance of Protease as a Cleaning Agent for Stainless Steel Surfaces Fouled with Protein
- Comparative Study on Caustic and Enzymatic Cleanings of Stainless Steel Surface Fouled with β-Lactoglobulin
- Adsorption of β-Lactoglobulin onto the Surface of Stainless Steel Particles
- The Production of Polyhydroxyalkanoate from Anaerobically Treated Palm Oil Mill Effluent by Rhodobacter sphaeroides
- On the Adsorption of Proteins on Solid Surfaces, a Common but Very Complicated Phenomenon
- Analysis of Monoglyceride Synthetic Reaction in a Solvent-Free Two-Phase System Catalyzed by a Monoacylglycerol Lipase from Pseudomonas sp.LP7315
- Purification and Characterization of a Monoacylglycerol Lipase from Pseudomonas sp.LP7315
- Characteristics of Serine Acetyltransferase from Escherichia coli Deleting Different Lengths of Amino Acid Residues from the C-Terminus
- Effects of Bienzyme Complex Formation of Cysteine Synthetase from Escherichia coli on Some Properties and Kinetics
- Kinetic Analysis of Synthesis of a Dipeptide Precursor Using an Immobilized Enzyme in Water-Immiscible Organic Solvents
- Kinetics and Equilibrium for Thermolysin-Catalyzed Syntheses of Dipeptide Precursors in Aqueous/Organic Biphasic Systems
- Development of Novel Advanced Oxidation System Using Combined UV/H_2O_2 Technique and Kinetic Analysis for Decomposition of Dye Solutions
- Analysis for Partition Equilibrium of Amino Acid Derivatives in Aqueous/Organic Biphasic Systems
- Formation of γ-Glutamylglycylglycine by Extracellular Glutaminase of Aspergillus oryzae
- γ-Glutamyltranspeptidase Activity and the Properties of the Extracellular Glutaminase from Aspergillus oryzae(Microbiology & Fermentation Industry)
- Establishment and Clinical Application of an Enzyme Immunoassay for Metastin to Determination of the Human Plasma Levels
- Production of Kojic Acid by Membrane-Surface Liquid Culture of Aspergillus oryzae NRRL484
- Production of Neutral Protease by Membrane-Surface Liquid Culture of Aspergillus oryzae IAM2704
- Chromatographic Separation of Galactosylkojic Acid
- Enzymatic Synthesis of Galactosylkojic Acid with Immobilized β-Galactosidase from Bacillus circulans
- Some Factors Affecting the Formation of γ-Cyclodextrin Using Cyclodextrin Glycosyltransferase from Bacillus sp. AL-6
- Purification and Properties of Cyclodextrin Glycosyltransferase from Bacillus sp. AL-6
- Systemic ATP infusion improves spontaneous pain and tactile allodynia, but not tactile hypesthesia, in patients with postherpetic neuralgia
- Lipase-Catalyzed Synthesis of Monolauroyl Maltose through Condensation of Maltose and Lauric Acid
- Condensation of L-Ascorbic Acid and Medium-Chain Fatty Acids by Immobilized Lipase in Acetonitrile with Low Water Content
- Development of Cylindrical Apparatus for Membrane-Surface Liquid Culture and Production of Kojic Acid Using Aspergillus oryzae NRRL484
- Effect of Nasal Continuous Positive Airway Pressure on Pulmonary Edema Complicating Acute Myocardial Infarction
- マレーシアにおけるパームオイル廃液を原料とする生分解性プラスチック生産の経済評価
- Synthesis of Peptides Consisting of Essential Amino acids by a Reactor System Using Three Proteinases and an Organic Solvent(Microbiology & Fermentation Industry)
- Enzymatic Synthesis of β-Lactam Antibiotics and N-Fatty-Acylated Amino Compounds by the Acyl-Transfer Reaction Catalyzed by Penicillin V Acylase from Streptomyces mobaraensis
- Filtration Characteristics and Structure of Cake in Crossflow Filtration of Bacterial Suspension
- Continuous Synthesis of a Tripeptide by Successive Condensation and Transesterification Catalyzed by Two Immobilized Proteinases in Organic Solvent(Microbiology & Fermentation Industry)
- FILTRATION BEHAVIORS OF ROD-SHAPED BACTERIAL BROTHS IN UNSTEADY-STATE PHASE OF CROSS-FLOW FILTRATION
- Purification and Characterization of Serine Acetyltransferase from Escherichia coli Partially Truncated at the C-Terminal Region
- Relationship between Cake Structure and Membrane Pore Size in Crossflow Filtration of Microbial Cell Suspension Containing Fine Particles
- 1P399 Suppression of ROS production by UCP2 in neuronal cell subjected to oxidative stress(15. Cellular signal transduction,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- Adsorption Characteristics of Various Proteins to a Titanium Surface(BIOCHEMICAL ENGINEERING)
- ATP Regeneration with Enzymes of the Alcohol Fermentation Pathway and Kinases of Yeast and Its Computer Simulation
- Stability of Immobilized Thermolysin in Organic Solvents
- Purification and Properties of a Cyclodextrin Glucanotransferase from Bacillus autolyticus 11149 and Selective Formation of β-Cyclodextrin
- Adsorption Characteristics of Various Proteins to a Titanium Surface
- CATALYST FOULING IN THE DEHYDROGENATION OF n-BUTANE OVER CHROMIA-ALUMINA CATALYSTS
- Desorption Behavior of Deposit Formed from Coffee Drinks on Stainless Steel Surfaces.
- GAS ABSORPTION WITH SECOND-ORDER CHEMICAL REACTION IN A PACKED COLUMN:EFFECTS OF CONTACT-TIME DISTRIBUTION
- Continuous CoA Production with Immobilized Brevibacterium ammoniagenes Cells
- Purification and Properties of β-Galactosidases from Bacillus circulans
- Mechanism of Ice Growth in a Batch Crystalljzer with an External Cooler for Freeze Concentration
- EFFECTIVE DIFFUSIVITY OF A POROUS CATALYST WITH AND WITHOUT CHEMICAL REACTION