Characterization of Peroxisomes in an n-Alkane-Utilizable Yeast, Candida tropicalis, Grown on Glucose and Propionate
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概要
- 論文の詳細を見る
Two types of peroxisomes (microbodies) in different states of development, which are attractive for investigating the biogenesis of the organelles, were first isolated from the glucose- and propionate-grown cells of an n-alkane-utilizable yeast, Candida tropicalis pK 233,and their properties were discloased. Peroxisomes isolated from the glucose-grown cells contained high levels of isocitrate lyase and malate synthase, whereas the levels of catalase and enzymes constituting constituting the fatty acid β-oxidation system were demonstrated to be very low by means of subcellular fractionation and Western blot analysis. Citrate synthase common to the tricarboxylic acid and glyioxylate cycles was localized only in mitochondrai. We have proposed that the peroxisomes in the glucose-grown cells are called 'pro-peroxisomes' or 'prooxisomes' to distinguish them from the 'mature' organelles in the alkane-grown cells which have high levels of catalase and the β-oxidation enzymes. The peroxisomes isolated from the propinate-grown cells exhibited higher levels of isocitrate lyase and malate synthase together with moderate levels of catalase and acyl-CoA oxidase inducibly synthesize in the cells. That is, the peroxisomes in the propionate-grown cells showed characteristics of both 'prooxisomes' and 'mature' peroxisomes, and were supposed to be an intermediate form deficient in some of the developmental processes.
- 公益社団法人日本生物工学会の論文
- 1989-12-25
著者
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Atomi H
Department Of Synthetic Chemistry And Biological Chemistry Graduate School Of Engineering Kyoto Univ
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Atomi Haruyuki
Laboratory Of Applied Biological Chemistry Department Of Synthetic Chemistry And Biological Chemistr
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UEDA Mitsuyoshi
京都大学大学院工学研究科
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Osumi Masako
Department Of Chemical And Biological Sciences Faculty Of Science Japan Women's University
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Osumi Masako
Department Of Chemical And Biological Sciences Faculty Of Science Japan Woman's University
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Tanaka Atsuo
Laboratory Of Applied Biological Chemistry Department Of Synthetic Chemistry And Biological Chamistr
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Ueda Mitsuyoshi
Laboratory Of Applied Biological Chemistry Department Of Synthetic Chemistry And Biological Chemistr
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MOZAFFAR SABIHA
Laboratory of Industrial Biochemistry, Department of Industrial Chemistry, Faculty of Engineering, K
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Osumi Masako
Department Of Biology Faculty Of Science Osaka City University
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Mozaffar Sabiha
Laboratory Of Industrial Biochemistry Department Of Industrial Chemistry Faculty Of Engineering Kyot
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